Abstract:
The experiment was conducted at the Plant Tissue Culture Center Trust Laboratory of
Proshika Plant Tissue Culture Trust (PPTCC), Koitta. Manikgonj, Bangladesh from
October, 2007 to June. 2008. For shoot proliferation, MS (Murashige and Skoog) and
White's media supplented with four PGRs (BAP,Kn, NAA,ADS) were used singly at
five concentrations (0.1, 1.0, 1.5, 2.0,
2.5
mgfL). BA? 2mg/L and Kn 1 and 2 mg/L
were used in MS and White's media for synergism. PGRs and organic supplement
viz., BAP, Kn, NAA, lB A, IAA and orange and tomato juice were added in
Linsmiaier and Skoog (LS) and Phytamax (PM) media For callus induction, 2,4-D
2mgIL was added in MS and White's media .For root formation, MS, LS, PM and
White's media with IBA 2mgIL was used. For plantlet etablishment. the proportion of
soil, sand and fertilizer /cowdung/poultry litter were used in 1:1 :1. MS media with 2.5
mg/L was the best shoot proliferation using single PGR For, combined etTect of PGR
and organic supplement, best result was found with PM media supplented with IBA
0.5 mg/L. For synergism, best result was found with MS media supplemented with
BA? mg/L with Kn I mg/L. Maximum root formation was observed with MS media
with IBA I mgfL. MS media with 2,4-D 2mgfL was the best for callus induction.
Combination of garden soil, sand and cowdung (1:1:1) was the best plantlet
establishment comparing other treatments.
