CLONING OF GINGER (Zigiber officinale) THROUGH IN VITRO TECHNIQUE

Abstract

The experiment was conducted . at the Proshika plant tissue culture Laboratory,Manikganj,Dhaka during the month of September 2007 to May 2008 to study Cloning of ginger through in vitro Techniques. Sprouted buds from rhizome of ginger (variety Thai) were cultured in four different basal medium with diifercnt hormone (NAA, IAA, BA, KN, RAP, IBA & ADS) and their combination to lind out suitable combination of media and also find out the best soil type for its successive growth and development. In case of single hormone NAA at (1mg/I) concentration with MS media showed highest shoot length (3.0cm), highest leaf number (2.2) and multiple tillers (2.2). In combined effect BA (2mg/I) and KN (1mg/I) with MS medium showed the best performance, with highest pseudostem length (1.4cm), liigicst leaf numbcr(2.4) and highest multiple tiller (2.0). In case of LS medium with organic supplement and hormone RAP with KN with tomato juice gave highest shoot length (5.0cm) with 78% of rooting. In case of rooting hormone IBA at (1mg/I) with MS and White media achieved 82% root. For soil, garden soil: sand: soil (I 1:1) showed highest growth rate (plant height 11 .Scni),It may he concluded that shoot induction was good in MS media and root induction was best in MS and \Vhite liquid rooting media.