INTRODUCTION OF SALT TOLERANT GENE IN WHITE JUTE THROUGH AGROBACTERIUM VECTOR

Abstract

An experiment was conducted at the Biotechnology Laboratory, Department of Genetic Resources and Seed Division. IIangladcsh Jute Research Institute. Dhaka during the period of march 2008 to February 2009 on white Jute (Corchorus capsulari.c) which is one of the world's most economical!)' important libcr crops. Genetic Engineering or recombinant DNA technology can resolve the various biotic and abiotic problems by making transgenic plant. The regeneration and transtbrmation processes depend on optimum growth conditions, suitable explants and varieties. An attempt was made for Agrobaclenum mediated genetic transformation in white jute varieties using gene construct conferring both salt and drought tolerance (CIPK and Glv-l)along with the marker genes. Interestingly the three varieties (CVL-1.CVF-3 and Tricap- I) showed the response of both callus induction and plant regeneration on a single formulation i.e. MS medium supplemented with 2.0 mg/I BAP and 0.5 mg/I IAA and cotyledons with attach petioles of 7-8 days old seedling .An efficient and reproducible protocol for transgenic jute plants was established by inoculating cotyledons with attached petioles and hypocotile with agrobcwierium turnefaciens strains LBA4404contains both salt and drought tolerant genes (CIPK and (fly-I with selectable marker gene Nptll conferring resistance to kanamycin and GUS reporter gene also. Explants were dipped to liquid culture of bacteria for one minute and then transferred to co-cultivation media for 24 hours. Shoot regeneration from Agrohacieriwn infected cotyledon was fotind highest in variety CVI.-1 than Trieap. After co-cultivation and selection histoehemieal GUS assay was performed in three varieties (vars. Tricap-l. CVL-1 and CVE-3). In the transformed explants. GUS reporter gene was expressed showing blue color in the explant tissues. Among the varieties CVE-3 Showed the highest expression blue color in the explant tissues. l'hose transgenic plants are transferred to salt medium and soil for trial.