IN VITRO REGENERATION OF BANANA CV. AMRITASAGAR AND SABRI THROUGH SHOOT TIP CULTURE

Abstract

The study was undertaken with a view to establish a protocol for in vitro culture and plant regeneration from shoot tip explants of banana cv. Amritasagar and Sabri. This experiment was setup at Proshika Tissue Culture Centre Trust (PTCCT) laboratory, Manikgonj during the period from April to November, 2008. In the present study, BAP (0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 mg/I) and ISA (0.0. 1.0, 2.0, 3.0, 4.0 and 5.0 mg/I) have been used in different concentrations on MS medium to assess the influence on in vitro shoot regeneration and subsequent root formation. Explants were sterilized by 0.1% I-lgCl2 for 14 minutes and cent percent shoot tip explants were found free from contamination after 10 days of sterilization and inoculation. Explants of Anritasagar and Sabri turned brown, light green, green and dark green in colour after ID days of inoculation but after 20 days all became dark green at all concentrations of BAP except control in Amritasagar and control and 1.0 mg/I in Sabri. The response of single shoot lbniiation in Amritasagar was the highest (50%) followed by Sabri (300/4) at 5.0 mg/I BAP within 10-15 days. Among the concentrations of BAP, 5.0 mg/I showed the best shoot proliferation and the lowest was at 1.0 mg/I. No response was observed at control. Shoot proliferation rate of Amritasagar was better than that of Sabri. The eultivar Amritasagar and Sabri produced 3.50 shoots/explant and 2.00 shoots/explant, respectively at 5.0 mg/I BAP at 30 DAI. Amritasagar also produced longer shoot (2.64 cm) compared to Sabri (2.16 cm) at 5.0 mg/I BAP at 30 DAI. Among the concentrations of iSA, 3.0 mg/I proved to be the best for root induction. Amritasagar showed superior performance (4.10 roots/plantict) for root induction over Sabri (3.63 roots/plantlet) at 3.0 mg/I IBA at 30 DAI. The survival of the plantlets of both eultivars tinder cx vitro condition was more than 82% and all of them resumed growth in the field.