Abstract:
Two section of experiment were conducted during the period From July 2006 to June
2007 in the Genetic Engineering Laboratoiy of Genetic Resources and Seed Division,
Bangladesh Jute Research Institute (BJRI), Dhaka. In this section-A, C. capsukiris was
used to investigate their in vitro regeneration potentiality and in the section-B, two
vaneties of C. capsularis were used to observe their transformation ability. Cotyledons
(with attached petioles) were used as explants. One of the major constraints of getting
plant regeneration from the explants of C. capsularis was the production of healthy
seedlings in vitro. Seeds of C. capsufaris germinated on both agar supported hormone
free MS medium and cotton supported hormone free liquid MS medium. The percentage
of seeds germinated on cotton-supported medium was found to be much higher than
seeds germinated on agar-supported medium. In the section-A, seed germination
percentage was found to be the highest in CVE-3 in cotton-supported medium (88.89%)
than agar-supported medium (77.77%). Among the phytohorntone combination, MS+ 2
mg/I HAl' 7 0.5 mg/I IAA showed the highest shoot regeneration (83.33%). l'lant
regeneration was also observed in the presence of different percentages of surfaetant
(Pluronic F-os) and different concentration of PeSO.1 and it was found that 0.08%
surfactant and 28 mg/I FeSO4 gave highest result respectively. In the section-B, an
efficient and reproducible protocol for the production of transgenie jute plant was
developed by inoculating cotyledonary petioles with Agroliacicriu,n lumefaciens strain
LBA4404 carrying a binary vector pBI12I, which contains selectable marker gene npuii
conferring resistance to kanamycin and the GUS reporter gene. After co-cultivation and
selection, histochemical GUS assay was performed in two varieties (viz. CVE3 and
C\'Ll). In the transformed explants, GUS reporter gene was expressed showing blue
colour in the explain tissues. Non-transformed explants did not show any colour. Among
the varieties, CVE-3 showed the highest response to GUS assay (90%).
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
GENETICS AND PLANT BREEDING
SEMESTER: JANUARY-JUNE, 2007
