Abstract:
Executive Summary
Cattle is one of most crucial genetic resources among the farm animal genetic resources
(FAnGR). Genetic improvement of cattle can be done by planned AI (Artificial
insemination), multiple ovulation and embryo transfer (MOET) and in-vitro production
(IVP) of embryos in Bangladesh. Among reproductive technologies most popular in
Bangladesh is AI. Due to AI, semen quality evaluation is important to selection. To efficient
calf good quality semen has positive effect on successful fertility of cows and heifer as well
as cattle breeding programme. We conducted this study to evaluate quality of both frozen
and fresh production of bull in Bangladesh. For the purpose of this study, both fresh and
frozen semen were collected from CCBDF (Central Cattle Breeding and Dairy Farm) Savar,
Dhaka. Fresh semen collected by artificial vaginal method (AV) at early morning. Then
sample transported through thermal box to laboratory. This study was conducted at
Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural
University, Dhaka-1207. After collection of semen by AV method from bull, frozen semen
straw were prepared and stored at -196
o
c in liquid nitrogen cane for AI purpose at CCBDF.
The frozen sample straw was collected and transported through N
cane to laboratory. To
assessment of morphometric characteristics of semen qualities, these parameters were
evaluated such as sperm concentration ×10
6
2
/ml, normal sperm, abnormal sperm, dead sperm,
live sperm, total motility and progressive motility. The fresh and frozen semen were
comparable and statistically significant (p<0.05). The abnormal sperm of fresh and frozen
semen were 9.98% and 18.2% respectively. The live sperm of fresh and frozen semen were
88.43% and 68.04% respectively. The dead sperm of fresh and frozen semen were 11.8%
and 46.3% respectively. The normal sperm of fresh and frozen semen were 87.3% and
65.09% respectively. The sperm concentration ×10
6
//ml of crossbred is 1370.00×10
/ml and
1223.00×10
6
/ml were fresh and frozen respectively. The progressive motility of fresh and
frozen semen were 75.26% and 44.32% respectively. The motility of fresh and frozen semen
were 85.93% and 62.16% respectively. To discard poor fertile bulls in AI programme, semen
quality evaluation is a crucial element. The fresh semen quality better than frozen semen.
But to avoid venereal diseases frozen disease straw better than fresh semen and effective to
get desirable calf per year. So, frozen semen should be undertaken with special management
to maintain maximum quality which can be used in the field level for genetic improvement.
