Abstract:
Sets of experiments were conducted in the Genetic lin2ineering Laboratory.
('vtogenetic Department, Bangladesh Jute Research Institute (BJRI ). DhaLa
during the period from March 2005 to May 2006 to lid fill the objectives of
the study. One of the major constraints of getting plant regeneralion from the
explants of C'. o/UOP1OILV was the production of healthy seedlings iii vitro.
Seeds of C'. o/iloriouc germinated on both agar supported hormone free MS
medium and cotton supported hormone free liquid MS medium. Ihe
percentage of seeds germinated on cotton supported medium was found to
be iiiuch higher than seeds germinated on agar supported medium. i'his
system ensured maximum of an average of 97.55% seed geriflinatioll.
Whereas, in case of agar supported medium an average of 45.8% seeds
could be germinated with very slow growth. The seedling grown on cotton
supported medium were found to much more healthy than seedlings growli
on agar supported medium. Plant regeneration obtained from die
cotyledonary petioles of the varieties of C'. olitorinus on MS agar solidi fled
medium supplemented by IAA 0.5 mg/I and I3AP 3.0 mg/I. Plant
regeneration was also observed in dil'lerent level of ph and dillerent
concentration of FeSO4 and it was found that 5.5 and 56 mg/I gave highest
result. Ihe efficiency of plant regeneration from the cotton supported
seedlings was found to be better as the agar supported seedlings. The
plantlets produced roots on hormone free MS mediuni rapidly. After translèr
to soil the plants grew into maturity and produced fruits. No morphological
changes were noticed.
Description:
A Thesis
Submitted to the Faculty of Agriculture.
Shcr-e-Bangla Agricultural University. Dhaka.
in partial rut lii Iment of the requirements
for the degree of
MASTER OF SCIENCE
IN
GENETICS AND PLANT BREEDING
SEMESTER: JANUARY-JUNE, 2006
