Abstract:
The present study introduces a UHPLC method that is simple, specific, and accurate
in measuring the active component imidacloprid in the 20SL formulation. The
analytical separation was conducted using a Waters Cortecs C18 column using
an ultraviolet photodiode array (UV-PDA) detector at a wavelength of 272 nm. The
method's validation results showed that the suggested method has good specificity
with satisfactory linearity (R
2
≥ 0.9994), short retention time, and peak area precision.
The method's accuracy and precision revealed a significant recovery (98.9 - 101.2%)
at the three-spiked level with excellent precision (less than 1.89% RSD). The
chromatographic separation took around 5 minutes to complete under the established
chromatographic conditions. The developed and validated method was used to
determine the purity of mostly used and available of seven (7) selected imidacloprid
marketed brands insecticide from twenty one (21) samples. The samples were
collected from sadar upazilla of Jessore district during September 2021 and then the
samples were carried to the food safety laboratory of the Sher-e-Bangla
Agricultural University, Dhaka on the same sampling day. Among 7 different
brands of imidacloprid insecticide samples, 5 (71.43%) were found 110-130% pure
which means over standard of active ingredient (AI). Whereas one of the total tested
brand was 80-90% pure, and the remaining one brand was below 10% pure. This
means that farmers are using imidacloprid having either higher or less of AI than
required. This study reflects the small scale scenario of the presence of AI in different
marketed brands of imidacloprid insecticide, which will help the policy maker to be
aware of quality control importance of the plant protection products.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
In partial fulfillment of the requirements for the degree of
MASTER OF SCIENCE
IN
AGRICULTURAL CHEMISTRY
