DNA FINGERPRINTING AND MOLECULAR DIVERSITY OF MUNG BEAN (Vigna radiata (L.) R. Wilczek) GERMPLASM

Abstract

Mung bean (Vigna radiata (L.) R. Wilczek) has been widely used as a pulse crop. The study was carried out at the Biotechnology laboratory of Sher-e-Bangla Agricultural University, during the period of July 2021 to June 2022 to investigate molecular diversity and fingerprinting of fifteen mung bean germplasm. RAPD primers were selected and synthesized for molecular diversity analysis. All of the primers were able to produce polymorphic bands. The highest number of bands were observed in RAPD primer OPT20.

The polymorphic Information Content (PIC) of RAPD markers ranged from 0.24 to 0.32 with an average value of PIC =0.27. Gene diversity ranges from 0.41 to 0.28 with an average value of 0.33. Primer showed OPBC-1, OPA-20, OPP-03, OPT-20 moderate diversity and primer OPBC 18 showed low diversity on the basis of PIC value. The value of pair -wise comparisons of Nei’s (1983) genetic distance (D) among 15 genotypes was computed from and it was ranged from 0.1034 to 0.6207 with an average of 0.3621. Dendrogram based on Nei’s genetic distance using unweighted Pair Group Method Of Arithmetic Mean (UPGMA) indicated the segregation of 15 genotypes into four main clusters. This result can be conveniently used for molecular diversity analysis to identify diverse parent for mung bean improvement program.