IN VITRO REGENERATION OF Aloe vera L. IN A NEW PLANT TISSUE CULTURE MEDIUM

Abstract

The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period from July 2021 to June 2022, to evaluate the regeneration of Aloe vera in a new tissue culture medium (SAU Tissue Culture Medium or Hoque medium) which was marked as Treatment-3. MS (1962) medium was used as Treatment-1 and MS Powder (Duchefa, The Netharland) was used as Treatment2.

It was observed that, the highest percent (90%) of shoot regeneration was observed in the SAU Tissue Culture Medium. The same treatment showed maximum number of shoot in all the study period. The highest number of leaves were found in MS powder (Duchefa, The Netherland) medium and it was lowest in MS (1962) medium. Longest length of root (7.00) cm was observed in SAU Tissue Culture Medium and it was shortage (5.10) cm in MS (1962) Medium. Those three treatments were supplemented with same dose of phytohormone (1.00mg/L KIN + 2.00 mg/L NAA) to find out the ability of media in respect of hormonal condition. It showed that there was no significant difference in respect of days to shoot initiation and percent of shoot regeneration among the SAU Tissue Culture Medium and MS powder (Duchefa, The Netharland) when supllimented with phytohormone. Number of shoot per explant and number of leaves also showed non-significant difference between the Treatment-3 (SAU Tissue Culture Medium) and Treatment-2{MS powder (Duchefa, The Netherland)}. Percent of root induction and length of root also showed similar result among the Treatment-2 and Treatment-3. The MS (1962) medium showed lowest result for all the parameter under studied. The study highlighted the potentiality of SAU Tissue Culture Medium on In vitro regeneration of Aloe vera. It has indicated that the newly developed SAU Tissue Culture Medium (Hoque Medium) has excellent result on plantlet regeneration in Aloe vera. Hence, the formulation of SAU Tissue Culture Medium is a successful innovation in the field of tissue culture technology which can be applied for any other plant species.