Abstract:
The experiment was conducted at the Biotechnology Laboratory, Department of
Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh
during the period from July 2021 to June 2022, to evaluate the regeneration of
Aloe vera in a new tissue culture medium (SAU Tissue Culture Medium or Hoque
medium) which was marked as Treatment-3. MS (1962) medium was used as
Treatment-1 and MS Powder (Duchefa, The Netharland) was used as Treatment2.
It was observed that, the highest percent (90%) of shoot regeneration was
observed in the SAU Tissue Culture Medium. The same treatment showed
maximum number of shoot in all the study period. The highest number of leaves
were found in MS powder (Duchefa, The Netherland) medium and it was lowest
in MS (1962) medium. Longest length of root (7.00) cm was observed in SAU
Tissue Culture Medium and it was shortage (5.10) cm in MS (1962) Medium.
Those three treatments were supplemented with same dose of phytohormone
(1.00mg/L KIN + 2.00 mg/L NAA) to find out the ability of media in respect of
hormonal condition. It showed that there was no significant difference in respect
of days to shoot initiation and percent of shoot regeneration among the SAU
Tissue Culture Medium and MS powder (Duchefa, The Netharland) when
supllimented with phytohormone. Number of shoot per explant and number of
leaves also showed non-significant difference between the Treatment-3 (SAU
Tissue Culture Medium) and Treatment-2{MS powder (Duchefa, The
Netherland)}. Percent of root induction and length of root also showed similar
result among the Treatment-2 and Treatment-3. The MS (1962) medium showed
lowest result for all the parameter under studied. The study highlighted the
potentiality of SAU Tissue Culture Medium on In vitro regeneration of Aloe vera.
It has indicated that the newly developed SAU Tissue Culture Medium (Hoque
Medium) has excellent result on plantlet regeneration in Aloe vera. Hence, the
formulation of SAU Tissue Culture Medium is a successful innovation in the field
of tissue culture technology which can be applied for any other plant species.
Description:
A thesis Paper
Submitted to the faculty of Agriculture, Sher-e-Bangla Agricultural
University, Dhaka in partial fulfillment of the requirements for the degree
of
MASTER OF SCIENCE
