Abstract:
A pot and a lab experiment was conducted to evaluate the efficacy of selected bioagents
(Metarhizium anisopliae, Trichoderma harzianum, Verticillum lecanii,
Beauveria bessiana), botanicals [Clerodendrum infortunatum (Vat) leaf extract,
Lantana camara leaf extract, Azadiracta indica (Neem) leaf extract] and agro bioproducts
(Tea
wastage,
Garlic
powder,
Mustard
oil
cake)
against
the
wilt
complex
and
Tomato yellow leaf curl virus (TYLCV) in summer tomato cultivation. The
experiment was conducted at net house in the Department of Plant Pathology, Shere-Bangla
Agricultural
University
(SAU),
Dhaka,
Bangladesh,
during
the
period
of
July
to
October,
2021.
The
experiment
was
carried
out
in
a
complete
randomized
design
(CRD)
with
three
replications
and
each
of
the
treatment
contains
3
pots.
The
total
number
of
unit
pots
was
30.
Summer
variety
‘BARI
hybrid
tomato-4’
was
used
as
a tested
cultivar.
Data
on disease
incidence
and disease
severity
of
wilt
complex
and
TYLCV
was
recorded
at
30,
45
and
60
DAT.
Fungal
pathogen
was
isolated
by
tissue
planting
method. Bacterial pathogen was isolated by dilution and streaking
method. In case of wilt complex, among the selected bio–agents, the lowest disease
incidence (33.33%) and severity (15%) was found in T
1
(Metarhizium anisopliae +
Trichoderma harzianum) and the highest disease incidence (66.67%) and severity
(20%) was found in T
3
(Metarhizium anisopliae + Beauveria bessiana). In case of
TYLCV, the lowest disease incidence (66.67%) and severity (20%) was found in T
3
(Metarhizium anisopliae + Beauveria bessiana) and the highest disease incidence
(100%) and severity (30%) was found in T
2
(Metarhizium anisopliae + Verticillum
lecanii). From the present study, it was found that the wilt complex of tomato
caused by Fusarium oxysporum f.sp. lycopersici and Ralstonia solanacearum that
was confirmed by morphological characteristics of macro and micro conidial
structures and biochemical tests viz. gram staining, motility, KOH solubility,
catalase and simon’s citrate test, respectively. The performance of the treatments in
respect of yield and yield contributing characters against wilt complex and TYLCV
of summer tomato varied significantly. However, from the present study it may be
concluded that mustard oil cake and neem leaf extract can be used as ecofriendly
approach for effective management of wilt complex and Tomato Yellow Leaf curl
Virus (TYLCV) in summer tomato cultivation.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
In partial fulfillment of the requirements for the degree
of
MASTER OF SCIENCE
IN
PLANT PATHOLOGY
