ISOLATION, IDENTIFICATION AND ANTIBIOTIC SENSITIVITY PROFILING OF ESCHERICHIA COLI AND SALMONELLA SPP. FROM DEAD BROILER IN LOCAL MARKET OF DHAKA CITY

Abstract

This study was carried out in Sher-e-Bangla Agricultural University, Dhaka for isolation, identification and antibiotic sensitivity profiling of Escherichia coli and Salmonella spp. from dead broiler in local market of Dhaka city. A total of 150 liver and heart samples were collected from dead broiler. The microorganisms of Escherichia coli and Salmonella spp. were isolated and identified. Samples were collected aseptically and inoculated onto various culture media for isolation of Escherichia coli and Salmonella spp. Identification of bacteria from positive samples was performed by cultural characteristics and biochemical tests. Hundred percent prevalence with microorganism was found from all the samples. The prevalence of E. coli and Salmonella spp. in liver samples was (45.33%) and (40%) and in heart samples it was (44%) and (38.67%) respectively. The overall prevalence of E. coli and Salmonella spp. in broiler was (44.67%) and (39.33%). Antibiotic sensitivity profiling of the isolated E. coli and Salmonella spp. was performed by the disc diffusion method against 10 commonly used antibiotics. The highest rate of sensitivity against E. coli was found with Ceftriaxone (52.24%) followed by Gentamicin (49.25%) and Streptomycin (44.78%). The highest rate of resistance was recorded in Tetracycline (52.24%) followed by Ampicillin (50.75%), Cefuroxime (47.76%), Amoxycillin (46.27%) and Co-Trimoxazole (46.27%). The highest rate of antibiotic sensitivity against Salmonella spp. was found with Ceftriaxone (52.54%) followed by Gentamicin (50.85%) and Streptomycin (47.46%). The highest rate of resistance of Salmonella was recorded in Cefuroxime (54.24%) followed by Ampicillin (52.54%), Cefixime (50.85%), Tetracycline (49.15%), Amoxycillin (47.46%) and Co-Trimoxazole (45.76%). Based on the present study, it may be concluded that Ceftriaxone should be best result against both E. coli and Salmonella spp. followed by Gentamicin and Streptomycin under the present study.