EFFECTS OF DIETARY SUPPLEMENTATION OF Sargassum vulgare ON GROWTH AND HEMATOLOGICAL PARAMETERS OF ORNAMENTAL FISH KOI CARP (Cyprinus rubrofuscus)

Abstract

A research was conducted to assess different formulated diets supplemented with different doses of seaweed (Sargassum vulgare) on ornamental fish Koi carp (Cyprinus rubrofuscus) in the aquaculture laboratory of Department of Aquaculture, Sher-e-Bangla Agricultural University to evaluate the growth and hematological parameters. Total four treatments were applied including Treatment- 1 (Control, 0% seaweed), Treatment-2 (5% seaweed), Treatment-3 (10% seaweed) and Treatment-4 (15% seaweed) each with three replicates. Four iso-proteinase feed was formulated where average percentage of protein, lipid, fiber and carbohydrate in formulated diet were 27.59%, 6.1%, 7.59 and 48.96%. The initial mean weight of fishes was 14.4±2.12, 14.5±0.75, 13.1±2.4 and 12.8±1.43 g in T-1, T2, T-3 and T4 respectively. The experiment was concluded in 100L glass aquarium with mechanized water filter system for each for 8 weeks and weekly sampling were done. Feed was applied twice a day. Temperature, pH, TDS, NH 3, NO 2 - ii

and NO 3 - data were collected daily during the experimental period and there was no significant difference in water parameters among treatments. Mean weight (g) of fish, length (cm) and width (cm) data were collected weekly basis. The mean weight gain was found 7.13±1.84, 8.06±1.58, 6.98±1.84 and 4.72±0.52 in T-1, T-2, T-3 and T-4 respectively. The highest weight gain was found in T-2 (5% seaweed) and the percentage of weight gain 55.86% found in the same treatment. There was no mortality found in T-2 and T-4. Survival rate were found 96.6% in Control. The lowest FCR 1.43±0.26 found in T-2 and SGR also found highest in the same treatment. Hematological parameter showed that blood hemoglobin and glucose level was higher in T-2 than others treatments which were 11.6±0.17gm/dl and 6.33±0.26 mmol/l. Serum protein was found 6gm/dl in T-2 and lowest value was found in control. Spleen Somatic Index (SSI) percentage of experimental fishes was also found higher in T-2 and lowest one was recorded in T-1. Refractive index (RI) of blood serum was found better in T-2 fishes compared to other treatments. It can be concluded that feed supplemented with 5% seaweed (T-2) diet showed better growth performance and hematological parameters compared to other treatments. Feed supplemented with brown seaweed Sargassum vulgare as a dietary ingredient can be used for better immunity and growth performance of ornamental fish.