Abstract:
A research was conducted to assess different formulated diets supplemented with
different doses of seaweed (Sargassum vulgare) on ornamental fish Koi carp
(Cyprinus rubrofuscus) in the aquaculture laboratory of Department of Aquaculture,
Sher-e-Bangla Agricultural University to evaluate the growth and hematological
parameters. Total four treatments were applied including Treatment- 1 (Control, 0%
seaweed), Treatment-2 (5% seaweed), Treatment-3 (10% seaweed) and Treatment-4
(15% seaweed) each with three replicates. Four iso-proteinase feed was formulated
where average percentage of protein, lipid, fiber and carbohydrate in formulated diet
were 27.59%, 6.1%, 7.59 and 48.96%. The initial mean weight of fishes was
14.4±2.12, 14.5±0.75, 13.1±2.4 and 12.8±1.43 g in T-1, T2, T-3 and T4 respectively.
The experiment was concluded in 100L glass aquarium with mechanized water filter
system for each for 8 weeks and weekly sampling were done. Feed was applied twice
a day. Temperature, pH, TDS, NH
3,
NO
2
-
ii
and NO
3
-
data were collected daily during the
experimental period and there was no significant difference in water parameters
among treatments. Mean weight (g) of fish, length (cm) and width (cm) data were
collected weekly basis. The mean weight gain was found 7.13±1.84, 8.06±1.58,
6.98±1.84 and 4.72±0.52 in T-1, T-2, T-3 and T-4 respectively. The highest weight
gain was found in T-2 (5% seaweed) and the percentage of weight gain 55.86% found
in the same treatment. There was no mortality found in T-2 and T-4. Survival rate
were found 96.6% in Control. The lowest FCR 1.43±0.26 found in T-2 and SGR also
found highest in the same treatment. Hematological parameter showed that blood
hemoglobin and glucose level was higher in T-2 than others treatments which were
11.6±0.17gm/dl and 6.33±0.26 mmol/l. Serum protein was found 6gm/dl in T-2 and
lowest value was found in control. Spleen Somatic Index (SSI) percentage of
experimental fishes was also found higher in T-2 and lowest one was recorded in T-1.
Refractive index (RI) of blood serum was found better in T-2 fishes compared to other
treatments. It can be concluded that feed supplemented with 5% seaweed (T-2) diet
showed better growth performance and hematological parameters compared to other
treatments. Feed supplemented with brown seaweed Sargassum vulgare as a dietary
ingredient can be used for better immunity and growth performance of ornamental
fish.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
SEMESTER: JULY-DECEMBER, 2021
