Abstract:
The experiment was conducted at the Biotechnology Laboratory of Department of
Biotechnology, Sher-e-Bangla Agricultural University, Dhaka from January 2021
to June 2021 to investigate the effect of different plant growth hormone for shoot
proliferation and root regeneration of Dendrobium sp. where shoot tips were used
as explant. The experimental data were taken on different parameters of shoot and
root growth. Different doses of individual shooting hormone(BA), individual
rooting hormone(NAA) and the combination of these two hormones were
experimented here. The minimum days to shoot induction were recorded 15 days
from treatment T
8
(2.0 mg/L BA + 1.50 mg/L NAA). Maximum percentage (73.4%)
of shoot induction was noticed in treatment T
8
(2.0 mg/L BA + 1.50 mg/L NAA)
.The maximum days to root induction was 44 days and it was recorded in control
treatment .The minimum days to root induction was 17 and it was observed from
treatment T
8
(2.0 mg/L BA + 1.50 mg/L NAA) days. In the following study
significant variation was observed for the use of BA and NAA on the percent of
shoot induction explants. Significant variation was observed for the use of BA and
NAA on the percent of root induction of explants . The treatment of 2.0 mg/L BA
response excellent in shoot induction and 1.0 mg/L NAA showed better
performance in root development in Dendrobium sp. Combined effect of BA and
NAA was very significant. Overall a reliable protocol was developed for shoot and
root proliferation which can be used for further crop improvement program.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfilment of the requirements
for the degree of
MASTER OF SCIENCE (MS) IN
BIOTECHNOLOGY
