Abstract:
In vitro bioassay of nine exotic potato genotypes namely CIP102, CIP106, CIP111, CIP
117, CIP 120, CIP124, CIP127, CIP 136 and CIP 139 was conducted for salinity
tolerance at Tissue Culture Lab, Tuber Crops Research Center (TCRC), Bangladesh
Agricultural Research Institute (BARI), Gazipur-1701. Single node and root tip
segments of these genotypes were cultured in MS media supplemented with 0.0
(control), 80, 100, 120, 140 and 160 mM NaCl. In case of in vitro shoot bioassay, CIP
139 was found as the most salt tolerant with the highest plant height (9.67 cm), number
of nodes (9.50), number of leaves (13.60), number of roots (8.00), length of root (6.50
cm), fresh weight of shoot (509.0 mg) and fresh weight of root (205.60 mg) at 160 mM
NaCl (14.61 dSm
). On the other hand, CIP 106 was found as the most salinity
sensitive at 120 mM NaCl (10.96 dSm
-1
) producing minimum plant height (7.17 cm),
number of nodes (6.50), number of leaves (12.50), number of roots (9.70), length of
roots (5.10 cm), fresh weight of shoot (572.3 mg) and fresh weight of root (244.4 mg)
followed by CIP 136, CIP 117 and CIP 111 at same salinity level. However, CIP 127,
CIP 102 and CIP 124 genotypes showed very good performance up to 140 mM NaCl
(12.78 dSm
-1
-1
). In vitro root bioassay also revealed the highest salinity tolerance of CIP
139 up to 160 mM NaCl, CIP 127 and CIP 102 up to 140 mM NaCl as well as CIP 106
up to 120 mM salinity level. The root tips of experimented potato genotypes were not
significantly affected up to 120 mM salinity level in comparison with control where CIP
120 was found as the lowest tolerant up to 120 mM salinity level in MS media. Among
the 0.0 (control), 0.1, 0.5, 1.0 and 1.5 mg /L IBA concentrations supplemented with MS
media 1.0 mg/ L was found best for rooting with early root initiation (6.57 days) and
well development (10.48 days), highest root length (8.63 cm), 2
highest root number
(17.25) and maximum fresh weight of root (332.22 mg). Interestingly, there was no
significant differences between 0.5 and 1.0 mg/L IBA for in vitro root induction and
development in the experimented CIP potato genotypes.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfilment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
Semester: January-June 2016
