ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI AND SALMONELLA FROM DRINKING WATER IN AND AROUND THE SAU CAMPUS

Abstract

ABSTRACT This study was conducted to investigate the E. coli and Salmonella from drinking water in SAU campus and its surrounding areas from January to August 2021. A total of 60 samples were collected from different halls, faculties and neighboring market areas of the SAU campus. Bacteria was identified observing the growth properties in different media, staining properties, microscopic observation and biochemical test. The characteristics colonies of E. coli were red to bright pink colored in MA agar & greenish red colored with faint metallic sheen in EMB agar. The characteristics colonies of Salmonella were black colored in SS agar & gray colored in EMB agar. Both bacteria observed in rod shaped, gram negative, single or paired arranged under microscope. In biochemical test both bacteria showed Catalase test, MR test positive and VP test negative. In case of Indole test E. coli showed positive but Salmonella showed negative indication. The higher prevalence (66.67%) was in Krishokrotno Sheikh Hasina Hall & Sheikh Sayera Khatun Hall. For faculties, the lower (33.33%) prevalence was in Sheikh Kamal Bhaban and higher (66.67%) was in Agriculture Faculty Bhaban. All samples collected from college gate street food shop were positive. The prevalence of E. coli & Salmonella in this study areas were 20% and 33%. The mixed contamination was 5%. So, direct consumption of water should not safe for us. It can cause serious health hazard.