IN VITRO PLANT REGENERATION OF Aloe vera L.

Abstract

The present research was carried out on Aloe vera L. in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e- Bangla Nagar, Dhaka-1207 during the period from September, 2013 to July, 2014 to evaluate the effect of different plant growth regulators on multiple shoot proliferation and root formation along with acclimatization for in vivo survival and finally to develop a viable micropropagation protocol. Shoot tip of young lateral shoots (sucker) were used as the explants, which were sterilized using freshly prepared 0.1% HgCl2 solution with few drops of Tween-20. After sterilization, explants were inoculated in MS media supplemented with either singly or in combination of the Benzylamino purine (BAP), Kinetine (KIN), Indolebutyric acid (IBA), Naphthaleneacetic acid (NAA). The highest percentage of shoot proliferation (85%) and minimum days to shoot induction (13.6 days) were achieved on MS medium containing 1.0 mg/L BAP + 0.5 mg/L IBA. The highest 14.8 shoots and 24.4 leaves per explant were also observed in the same media composition. But the highest average length of shoots (9.62 cm) was recorded from media containing 1.5 mg/L BAP + 1.0 mg/L NAA. The highest percentage (95%) of rooting was obtained in media treated with 3.0 mg/L IBA along with maximum 12.4 roots per explant. But the highest (10.24 cm) average length of root was observed in 2.0 mg/L IBA. The highest (5.374 mm) diameter of root and minimum days to root induction (10.6 days) were noticed on the same culture media supplemented with 2.5 mg/L NAA. In regenerated plantlets, 100% survival in growth chamber conditions and 88% survival under shade house and 90% survival in open atmosphere were achieved. Micropropagation was found to be very effective and promising method in the propagation of Aloe vera and this protocol can be a useful tool for proliferation of Aloe vera.