ASSESSMENT OF VARIETAL PERFORMANCE OF SELECTED TOMATO VARIETIES AGAINST Tomato Yellow Leaf Curl Virus (TYLCV) AND ITS MOLECULAR DETECTION THROUGH PCR

Abstract

An experiment was conducted in field and laboratory condition under the Department of Plant Pathology of Sher-e-Bangla Agricultural University, Dhaka-1207 during 2018-2020.The field experiment was carried out in a Randomized Complete Block Design (RCBD) with three replications. Genomic DNA extraction and PCR test was done in Molecular Biology and Plant Virology Laboratory following the standard protocol. In this experiment ten selected variety was selected for conducting the experiment. Among the ten selected varieties three varieties; BARI Tomato-5, BARI Tomato-18 & BARI Tomato-19 showed moderately resistant reaction and lower disease severity against the TYLCV. Remaining selected varieties; BARI Tomato-17, BARI Tomato-8, BARI Tomato-9, BARI Tomato-11, BARI Tomato- 14, BARI Tomato-15, BARI Tomato-16 were showed susceptible reactions to highly susceptible and higher disease severity against TYLCV. In case of BARI Tomato-5, BARI Tomato-18 & BARI Tomato-19 whitefly association was not increased with the increase of plant age. But it was noticed that in case of the remaining varieties whitefly association was increasing with the increase of plant age. Different growth parameters yield and yield contributing characters were also studied. Among the selected varieties the maximum number of leaves per plant was obtained in the variety BARI Tomato-11 and minimum was obtained in the variety BARI Tomato-9. The highest number of branches per plant was recorded in the variety BARI Tomato-11 and the lowest was recorded in the variety BARI Tomato-14. The maximum number of flowers was also obtained from BARI Tomato-11 and the minimum was observed from BARI Tomato-17. The maximum number of fruits was obtained from BARI Tomato-11 and the minimum was obtained from BARI Tomato-18. The maximum individual fruit weight was obtained from BARI Tomato-17 and minimum fruit weight was obtained from BARI Tomato-11. The highest yield per plant was obtained from BARI Tomato-17 and the lowest yield per plant was obtained from BARI Tomato-5. From molecular study through PCR test, it was revealed that results obtained on the basis of biological properties that was almost similar to PCR analyses to detect the TYLCV. Among the selected varieties, seven varieties; BARI Tomato-8, BARI Tomato-9, BARI Tomato-11, BARI Tomato-14, BARI Tomato-15, BARI Tomato-16 & BARI Tomato-17 gave the positive results in PCR test and shown sharp band at 520 bp fragment. Other varieties; BARI Tomato-5, BARI Tomato-18 and BARI Tomato-19 gave negative result in PCR test. For the molecular detection of Tomato Yellow Leaf Curl (TYLCV), PCR is the most reliable modern technique because it is simple, very specific and highly robust.