INTERSPECIFIC CROSS COMPATIBILITY STUDY BETWEEN Luffa acutangula AND Luffa cylindrica AND MOLECULAR ASSESSMENT OF HYBRIDITY IN F 1 GENERATION

Abstract

An experiment was carried out to study of the cross compatibility between ridge gourd and sponge gourd and assess hybridity in F generation through SSR marker. Field experiment was conducted at the farm of Sher-e-Bangla Agricultural University and laboratory experiment was conducted at Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka1207,

Bangladesh during the period of March 2017 to June 2018. Seven ridge gourd and three sponge gourd genotypes were used as parents. Most of the crosses were compatible such as Bogra×BARI Jhinga-1, Bogra×RIG 47-2, Faridpur×RIG 19-2 etc. and some crosses are incompatible such as Lalmonirhat×BARI Jhinga-1, Lalmonirhat×RIG 19-2 etc. Cross compatibility was maximum 75% in Bogra×BARI Jhinga-1. Fruit size and weight of the compatible crosses were intermediate between ridge gourd and sponge gourd. The interspecific hybrids produced fruits that have less number of seeds with less fiber density than that of the L. cylindrica entries. For hybridity test, initially different SSR primers were used to establish polymorphism among the parents of each hybrid. The identified polymorphic primers were used to screen the hybridity test. It was noticed that three primers viz. BoG-21, BoG-76 and BoG-117 showed polymorphism in the parents and their corresponding hybrids. It was noted that, a 550bp DNA fragment was amplified in male parent and hybrid of Bogra×RIG 47-2 which was absent in female genotype. Again, 200 bp DNA fragment was amplified in male parent and Bogra×BARI Jhinga-1 hybrid. This DNA band can be used for large scale screening of that specific hybrid variety for seed purity in commercial purpose. The parents of one hybrid (Faridpur×KRG 02) showed monomorphic DNA band and one of the SSR primers viz. BoG 118 was not amplified in any ridge gourd and sponge gourd genotypes.

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