IN VITRO REGENERATION OF PEPPER (Capsicum annuum L.)

Abstract

The present study was undertaken in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka1207 during the period from February, 2017 to March, 2018 to evaluate

the effect of different plant growth regulators (BAP and IBA) on in vitro plantlet regeneration of pepper. The shoot tips of pepper were used as explants which were inoculated in MS media supplemented with different concentration of BAP (1.0, 2.0, 3.0 and 4.0 mg/L) and IBA (1.0, 1.5, 2.0 and 2.5 mg/L) alone or in combination for plantlet regeneration. The experiments were arranged in CRD with three replications. The highest frequency (72%) of shoot development was observed in MS medium augmented with BAP 3.0 mg/L at minimum (18.25) days. Maximum number of shoots 3.50 mg/L was found in 35 days after shoot induction (DAI) at 3.0 mg/L BAP where the control treatment gave the lowest result. In combined effect, BAP 3.0 mg/L + IBA 2.0 mg/L showed the highest percentage (78.00%) of callus induction at in 16.00 days and the maximum size of callus (3.26 cm) was noticed at 35 days after induction. The highest number of shoot (3.76) was noticed from the same treatment at 35 DAI. The highest number of root (20.32) at 35 DAI was obtained in 2.0 mg/L IBA. The maximum length of roots (4.25 cm) was observed in 1.5 mg/L IBA

Finally, the survival rate of in vitro regenerated plantlets was recorded 75% in natural condition. Feasible protocol has been developed for in vitro rapid regeneration of pepper having potential in cell culture technology.