DETECTION AND IDENTIFICATION OF SEED BORNE BACTERIA OF MAIZE AND THEIR MANAGEMENT WITH SELECTED Cu-FUNGICIDES

Abstract

Approved by: Nazneen Sultana Chairman Examination Committee Department of Plant Pathology Sher-e-Bangla Agricultural University, Dhaka Nazneen Sultana Associate Professor Department of Plant Pathology Supervisor Mrs. Nasim Akhtar Professor Department of Plant Pathology Co-Supervisor Ref:………… Date:……………. CERTIFICATE This is to certify that thesis entitled, “DETECTION AND IDENTIFICATION OF SEED BORNE BACTERIA OF MAIZE AND THEIR MANAGEMENT WITH SELECTED Cu-FUNGICIDES” submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE in PLANT PATHOLOGY, embodies the result of a piece of bona fide research work carried out by KADAMBARI ROY, Registration No: 05-01829 under my supervision and guidance. No part of the thesis has been submitted for any other degree or diploma. I further certify that such help or source of information, as has been availed of during the course of this investigation has been duly been acknowledged by her. Nazneen Sultana Associate Professor Department of Plant Pathology Sher-e-Bangla Agricultural University Dhaka-1207, Bangladesh Email: nazneensau @yahoo.com Cell No.01733955171 ……………………………….. ( NAZNEEN SULTANA ) Supervisor Dated: 20th September, 2012 Dhaka, Bangladesh DEDICATED TO MY BELOVED PARENTS ACKNOWLEDGEMENT First and foremost the author would like to thank Almighty God for what he has given to the author and whose abundant grace and mercy has enabled for successful completion of the research, preparation of manuscript its submission in time as a partial requirement for the degree of MS (Master of Science) in Plant pathology. And after that the author expresses her greatful respect, wishes, whole hearted gratitude and appreciation to her benevolent teacher and supervisor Associate Professor and Chairman Nazneen Sultana, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, for her precious suggestions, constructive criticism, proper guidance and helpful comments through out the study. The author expresses with a deep sense of respect to her Co-supervisor Mrs. Nasim Akhtar Professor, Department of plant Pathology, Sher-e-Bangla Agricultural University, Dhaka for her cordial inspiration, guidance and helpful suggestions for its improvement. Her scholastic supervision and constant inspiration brought this thesis up to its present standard. Cordial thanks and honors to Dr. Md. Rafiqul Islam, Professor, Department of plant Pathology, Sher-e-Bangla Agricultural University, Dhaka for his valuable advice, criticism, suggestions and provision of facilities and support needed to undertake this research work. The authoress expresses her sincere appreciation and gratitude to her respectable teachers, Professor Dr. M. Salauddin M. Chowdhury and Associate Professor Dr.F.M. Aminuzzaman Department of plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, for their inspiration and encouragement throughout the whole period of the research work. The author also conveys her special thanks to Shanjida Haque, Nargis Islam Roni, Mamun-or-Rashid and Matin Sarkar for their cordial co-operation whenever required. The author is greatful to the office staffs of the Department of Plant Pathology for their co-operation and help. The whole credit for the achievements goes to the author’s family and highly gratitude to her great father Late Upendra Nath Roy, mother Jothsna Roy, sister and brother who always stood by her during tough times. They supported, encouraged her continuously to study and blessing in all phases of her academic life; they were motivators from near or far. They were her strength and their constant encouragement was an inspiration. June, 2011 Place: SAU, Dhaka The Author DETECTION AND IDENTIFICATION OF SEED BORNE BACTERIA OF MAIZE AND THEIR MANAGEMENT WITH SELECTED Cu-FUNGICIDES BY KADAMBARI ROY REGISTRATION NO. : 05-1829 ABSTRACT The experiment was carried out in the Department of Plant Pathology of Sher-e-Bangla Agricultural University, Dhaka during the period of January, 2012 to August, 2012 to determine the prevalence of seed borne bacteria of hybrid maize variety NK-40 and their management with some selected Cu-fungicides. Three bacterial genera viz. Acidovorax sp, Burkholderia sp and Ralstonia sp were isolated from maize seeds. Moreover, three Cu-fungicides viz. Sulcox 50WP, Champion 50WP and Cupravit 50WP were used as treatments for management. Bioassay of these fungicides against different bacteria were done and observed that Sulcox 50WP @ 0.3%, Champion 50WP @ 0.4% and Cupravit 50WP @ 0.3% produced remarkable inhibition zone against Acidovorax sp, Burkholderia sp and Ralstonia sp. Effect of seed treatments were studied using nutrient agar plate method, water agar test tube method and rolled paper towel method. In nutrient agar plate method, germination varied from 94.83-98.67%, where the effect of Cu-fungicides was insignificant. In water agar test tube method, the highest number of normal seedlings (69.00%) were recorded when seeds were treated with Cupravit 50WP @ 0.3% and the lowest was recorded in control (44.67%). The lowest number of abnormal seedlings and diseased seedlings (20% and 5%, respectively) were recorded when the seeds were treated with Cupravit 50WP @ 0.3%. In rolled paper towel method, Cupravit 50WP @ 0.3% showed best performance regarding germination, shoot length, root length and vigor index. The vigor index varied from 885.50-2488.40, where the highest count was recorded in Cupravit 50WP @ 0.3% and lowest in control.