Abstract:
The present research was carried out in Biotechnology Laboratory of the Department
of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207 from the
period of September 2013 to July 2014 to evaluate the effects of different plant
growth regulators on in vitro propagation of ginger (Zingiber officinale). The healthy,
disease free sprout was used as explants. Explants was inoculated in MS (Murashige
and Skoog), media supplemented with the Kinetine (KIN), alone and in combination
with GA3 and Indolebutyricacid (IBA). The highest percent of shoot induction (90%)
was observed on 3.0mg/L+1.5mg/L GA3 and the minimum days to shoot induction
(13.00 days) were achieved on KIN 3.0mg/L.The highest number of shoot (4.6),
maximum number of leaf per explants (10.00) and average length of shoot (4.56 cm)
were recorded in 3.0 mg/L KIN+ 1.5 mg/L GA3.The highest percent of root
induction 95% and the maximum (14.2) cm length of root induction were noticed on
2.5 mg/L IBA. Micropropagation was found very effective and promising method in
the proliferation of ginger and can be used for large scale production of disease free
and quality planting material.
Description:
the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
SEMESTER: JULY-DECEMBER, 2014
