MORPHOMETRIC ASSESSMENT OF OOCYTES RECOVERED FROM OVARIES OF BLACK BENGAL GOAT

Abstract

Ovary plays an important role in the reproductive biology and biotechnology of female animals. With the aim to study the oocyte morphometry of Black Bengal goat, both right and left ovaries were collected from the slaughter houses of Dhaka city and the research was conducted at Animal Production and Management lab of Sher-e-Bangla Agricultural University. Ovaries were separated from the surrounding tissues, washed thoroughly with distilled water repeatedly and after that washed with normal saline. For each of the samples, gross parameters such as weight, length and width were recorded. The study revealed that the weights of right and left ovary were 0.79±0.17 g and 0.77±0.10 g, respectively. The length of right ovary was 1.36±0.21 cm and 1.39±0.32 cm of left ovary. The widths of right and left ovary were 1.00±0.13 cm and 0.98±0.15 cm, respectively. Oocytes were collected by three methods like dissection, slicing and aspiration. The result showed that slicing methods yield more oocytes with a moderate quality while aspiration methods yield a moderate oocytes count with good quality. The oocyte diameter was 100.21±4.84 µm and cumulus cells (CCs) diameter was 49.77±12.62 µm. The research revealed that the oocytes with larger diameter represent larger CCs diameter as well as good quality for in vitro production. These results will be helpful to manipulate ovarian functions, reproductive biology and biotechnology such as in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro embryo production (IVP) in small ruminants.