Abstract:
Ovary plays an important role in the reproductive biology and biotechnology of
female animals. With the aim to study the oocyte morphometry of Black Bengal
goat, both right and left ovaries were collected from the slaughter houses of Dhaka
city and the research was conducted at Animal Production and Management lab of
Sher-e-Bangla Agricultural University. Ovaries were separated from the
surrounding tissues, washed thoroughly with distilled water repeatedly and after
that washed with normal saline. For each of the samples, gross parameters such as
weight, length and width were recorded. The study revealed that the weights of
right and left ovary were 0.79±0.17 g and 0.77±0.10 g, respectively. The length of
right ovary was 1.36±0.21 cm and 1.39±0.32 cm of left ovary. The widths of right
and left ovary were 1.00±0.13 cm and 0.98±0.15 cm, respectively. Oocytes were
collected by three methods like dissection, slicing and aspiration. The result
showed that slicing methods yield more oocytes with a moderate quality while
aspiration methods yield a moderate oocytes count with good quality. The oocyte
diameter was 100.21±4.84 µm and cumulus cells (CCs) diameter was 49.77±12.62
µm. The research revealed that the oocytes with larger diameter represent larger
CCs diameter as well as good quality for in vitro production. These results will be
helpful to manipulate ovarian functions, reproductive biology and biotechnology
such as in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro embryo
production (IVP) in small ruminants.
Description:
A Thesis
Submitted to the Department of Animal Production and Management,
Animal Science and Veterinary Medicine Faculty,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of MASTER OF SCIENCE (MS)
IN ANIMAL SCIENCE
SEMESTER: JANUARY- JUNE, 2019
