Abstract:
The present research work was undertaken to isolate and to investigate the prevalence
of zoonotic bacteria from broiler meat samples sold in Krishi Market, Bihari Camp,
Agargaon Bazar, Taltola Bazaar and SAU Mini Bazaar, Dhaka, Bangladesh from
January to May 2018. A total of 45 samples were randomly collected from markets
and transported to the Microbiology and Parasitology laboratory of Sher-e-Bangla
Agricultural University for microbiological analysis. After processing of samples
primary culture was done in nutrient broth and nutrient agar then pure culture was
obtained from different selective media. The prevalence of Escherichia coli,
Salmonella spp. and Staphylococcus spp. were 74%, 42% and 18% respectively. All
pure isolates were subjected to Antibiogram assay test by disc diffusion method
against 8 different antibiotics. E. coli isolates were sensitive to ciprofloxacin (91.6%),
gentamycin (87.5%), azithromycin (66.66%), tetracycline (58%) and resistant to
penicillin (79.16%) , amoxycillin (75%), streptomycin (75%) and ampicillin (58.3%).
Among all Salmonella spp. isolates, ciprofloxacin (81.81%) showed the highest
susceptibility pattern followed by the gentamycin (72.72%) and azithromycin
(63.63%). Highest resistant pattern was showed by tetracycline (58%),
streptomycin(72.72%), penicillin (72.72%) and amoxycillin (63.63%). In case of
Staphylococcus spp., ciprofloxacin (85.71%) showed the highest susceptibility pattern
followed by the gentamycin (71.42%). also, ampicillin (71.42%), streptomycin
(57.14%), azithromycin (57.14%) and tetracycline (57.14%) found sensitive in this
study. Highest resistant pattern showed by amoxycillin (71.42%) and penicillin
(71.42%). This study revealed that broiler meat sold at some local markets in Dhaka
city were contaminated with multiple species of multidrug resistant bacteria which
may risk for human health.
