DNA MARKER BASED HYBRIDITY TEST OF DIFFERENT EGGPLANT (Solanum melongena L.) HYBRIDS

Abstract

An experiment was carried out to identify the hybrid variety of eggplant through SSR marker. Four eggplant hybrids vizMEH5003, MEH5006, MEH5009, MEH5012 and their corresponding parents were used as experimental materials. The hybrid varieties of eggplant were developed by Metal Seed Ltd., Dhaka, Bangladesh. The experiment was conducted at the Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Initially different SSR primerswere used to establish polymorphism among the parents of each hybrid. The identified polymorphic primers were used to screen the hybrid variety together with corresponding parents. It was noticed that only one primer viz MEH SSR-04 showed polymorphism in the parents of MEH5009 hybrid. This primer was used for the detection of hybrid variety. A 150bp DNA fragment was amplified in male parent and hybrid variety which was absent in female genotype. This DNA band can be used for large scale screening of that specific hybrid variety for seed purity in commercial purpose. The parents of other hybrid showed monomorphic DNA band and some of the SSR primerswere not amplified in any eggplant genotype under investigation. The confirmation of genetic purity in seeds is very important for identification of genetic contamination and hybrid purity for farmers and plant breeders before sale of the seed to public domain. The genetic purity test by conventional method called Grow Out Test