IN VITRO REGENERATION OF DENDROBIUM ORCHID WITH DIFFERENT MEDIA AND PGRs FROM PROTOCORM LIKE BODIES

Abstract

An experiment was conducted at the Horticulture Farm of Sher-e-Bangla Agricultural University, Dhaka during the period from August to October 2006 to study the effect of different levels of nitrogen viz. control (N

0 ), 50 kg N per hectare (N ), 75 kg N per hectare (N 2 ), and 100 kg N per hectare (N 3 ) as well as different levels of P 2 O 5 viz. control (P

per hectare (P 1 ), 75 kg P 2 O 5 per hectare (P 2 ) and 85 kg P 2 O 5 per hectare (P ) on the growth and yield of Gimakalmi. The experiment was conducted in the Randomized Complete Block Design (RCBD) with three replications. Application of different levels of nitrogen significantly influence the growth and yield of Gimakalmi. The maximum (20.00g) fresh weight of leaves per plant was obtained from N 3 3 treatment while the minimum (18.66g) was found from control treatment (N ) at 60 days after sowing. The highest (8.27g) dry weight of leaves per plant was observed in N and the minimum (6.90g) was recorded from control treatment. The maximum (20.25g) fresh weight of leaves per plant was obtained from P 3 treatment and minimum (18.00 g) of fresh weight per plant was obtained from P treatment. The combined effect of various levels of nitrogen and P 2 O 5 0 were also found significant incase of gimakalmi production. The highest yield (62.66 t/ha) was performed from the treatment combination in N 3 P . The lowest yield (55.00t/ha) was obtained from the control treatment where no nitrogen and P 2 O 5 3 were used. The benefit cost ratio (BCR) was maximum (3.00) in the treatment combination of N 3 P 3 whereas the minimum (2.13) was recorded from the control treatment (N