Abstract:
An experiment was conducted at the Biotechnology Division, Bangladesh
Agricultural Research Institute (BARI), Joydebpur, Gazipur on In vitro
Regeneration of Ginger tZingiber officinale), The experiment was undertaken
to find out the suitable combination of plant growth regulators with appropriate
concentration for successful in vitro propagation of ginger from shoot tip on
MS media. The explant of one genotype of ginger was cultured in MS medium
with different concentrations and combinations of NAA (0. 1.0, 2.0, 3.0, 4.0
mg/L) and BAP (0, 1.0, 2.0, 3.0. 4.0 mg/L) to observe the performance of
regeneration. The rate of shoot multiplication was varied in different treatment
combinations. The highest number of multiple shoots (7.10) was recorded in
1.0 mg/L AA + 3.0 rng/L SAP. It was the lowest (2.42) in MS medium
without growth regulators. The maximum number of roots (9.61) and vigorous
roots was obtained in 1.0 mg/L NAA + 3.0 mg/L BAP. Days required for
shoots initiation is also influenced by NAA and BAP with different
combinations. The highest number of leaves (5.72) was found in 1.0 mg/L
AA + 4.0 mg/L BAP. The minimum time (20.67days) required for shoot
initiation was obtained in 1.0 mg/L NAA + 3.0 mg/L BAP.
