http://localhost:8080/handle/123456789/3611 Sat, 18 Apr 2026 17:38:25 GMT 2026-04-18T17:38:25Z http://localhost:8080/handle/123456789/3613 ISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM BOVINE RAW MILK SAMPLES IN AND AROUND THE DHAKA CITY SIKDER, TAHMINA Food-borne zoonotic hazards posed in milk and milk products are currently a serious concern of public health safety issue that plays a significant role on the emerging economies across the world especially in the low and middle income countries including Bangladesh. Raw or unpasteurized milk supports to be the excellent medium for a variety of bacterial growth, and transmits a number of bacterial pathogens cause diseases in consumers because of poor handling and also informal milk value chains. Many Previous studies reported that a number of bacterial pathogens were isolated from milk and milk products, and also from farms environments. Among these organisms, the most common pathogenic bacterial species in milk (raw) samples are Escherichia coli, Salmonella spp. Staphylococcus aureus, etc. which are the major public health concerns. The number of raw milk outlets has been continuously increasing in the local markets. These outlets retail milk to the poor people who are at high risk of the illnesses from milk-borne pathogens. So, milk could have the major public health concerns due to poses of the pathogenic bacteria for the communities who still consume raw or unpasteurized milk. Therefore, the current study conducted on a study to isolate and identify the pathogenic bacteria in bovine raw milk samples. A total of 54 bovine raw milk was collected and subjected to bacteriological analysis. In results, three bacterial species such as E. coli, Salmonella spp. and S. aureus were contaminated raw milk with a prevalence rate of 20.37%, 3.70% and 35.18 %, respectively. Besides, a high number of antibiotics were found to be resistant against all these 3 bacterial species, and the overall prevalence of MDR isolated bacteria was of 27.27%, 50% and 42.11% for E. coli, Salmonella spp. and S. aureus, respectively, which is indicated the serious public health concern. Based on the results demonstrated here, it could be concluded that proper hygienic maintenance of both milking and farm husbandry practices might reduce the chances of bacterial contamination. Besides, it is highly suggested that there is the urgency of increase adequate public awareness about the importance of hygienic milk production and also consumption of pasteurized/ processed milk to prevent milk borne infections. A Thesis Submitted to the Department of Pathology Sher-e-Bangla Agricultural University, Dhaka in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) PATHOLOGY Wed, 01 Jan 2020 00:00:00 GMT http://localhost:8080/handle/123456789/3613 2020-01-01T00:00:00Z