http://localhost:8080/handle/123456789/2764 Sat, 18 Apr 2026 16:16:02 GMT 2026-04-18T16:16:02Z http://localhost:8080/handle/123456789/693 ISOLATION AND CHARACTERIZATION OF MAINTAINERS AND RESTORERS FROM INTER SUB- SPECIFIC (indica xjaponica) DERIVATIVE LINES OF RICE BHUIYA, ARMIN The study was conducted at the experimental farm of Sher-e-Bangla Agricultural University, Dhaka-1207, during Kharif 2006, Rabi 2006-2007 and kharif 2007 seasons with the objective of identification of potential restorers and maintainers from indica/japonica derivative lines and conversion of selected maintainers possessing desirable plant type into CMS lines. To achieve the objectives, 65 test cross combinations including one wild abortive cytoplasmic male sterile (CMS) lines and 46 pollen parents were used as material of the study. The aspects of the study were development of source nursery and test cross nursery, estimation of pollen fertility, identification of potential restorers and potential maintainers. The study showed that of all the pollen parents in 65 success test crosses, 14 were restorers (21.54%), 27 were partial restorer (41.54%), 14 were partial maintainers (21 .54%) and 10 were maintainers (15.38%). Out of 14 restorer lines, 11 were selected on the basis of crop uniformity and all types of observation were recorded only from uniform lines. Based on scale of different characters a combined score was computed and on the basis of pooled approach of ranking, five restorers having the rank I to Ill will be used for production of experimental hybrid in next season. The 10 lines identified as maintainers were further subjected to screening based on various morphological characters favouring out-crossing, increased seed production, good grain quality as well as high level of heterosis. Following various scoring parameters and overall rankings four maintainer lines identified which were chosen for conversion into new CMS lines in the next crop season. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JANUARY-JUNE, 2008 Sun, 01 Jun 2008 00:00:00 GMT http://localhost:8080/handle/123456789/693 2008-06-01T00:00:00Z http://localhost:8080/handle/123456789/692 VARIABILITY, CORRELATION AND PATH ANALYSIS IN F3 MATERIALS OF Brassica rapa HOSEN, MONIR A research was conducted by using live parental genotypes of l3ra.tcica rupa and their ten F3 progenies including reciprocals in the experimental thmi of Sher-e-Bangla Agricultural University. Dhaka. during November 2006-March 2007 to study the variation in different characters. correlation between pairs of characters and the direct and indirect effect of different characters on seed yield per plant of the F3 matenals. From the values of mean. range and cv (%) of seed yield and yield contributing characters it was conlirmed that there were large variation present among the all genotypes used in the experiment. The values of phenotvpic variances was higher than the corresponding genotypic variances. Number of primary branches per plant, number of secondary branches per plant. days to 50% Ilowcnng. length of siliqua. number of seeds per siliqua. thousand seed weight and yield per plant showed least dit'Ibrence between phcnotvpic and genotypic variances. The values of GC'V and PCV indicated that there were considerable variation among the all characters except days to maturity. ftc plant height, days to 50% flowering and number of siliquae per plant showed high heritability with high genetic advance and genetic advance in percentage of mean. Fifteen most promising plants with yellow seed and short a duration from six crosses and lilly plants with brown seed and a short duration from eight crosses were selected out of the ten crosses of- the F materials of Ilrwsica rapa. Yield per plant had signilicant and highest positive correlation with thousand seed weight and yield per plant showed also positive correlation with days to maturity lollowed by number of seeds per siliquac, number of secondary branches per plant. length of siliqua and number of siliquae per plant. The path co-efficient analysis exhibited that thousand seed weight had the highest positive direct effect followed by days to 50% flowering, length of siliqua. number of primary branches per plant. number of secondary branches per plant. days to maturity and number of seeds per siliqua. A Thesis Submitted to the faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in partial fblfillment of the requirements for the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JANUARY- JUNE, 2008 Sun, 01 Jun 2008 00:00:00 GMT http://localhost:8080/handle/123456789/692 2008-06-01T00:00:00Z http://localhost:8080/handle/123456789/691 CHARACTERIZATION AND GENETIC DIVERSITY OF SPONGE GOURD (Luffa cylindrica L.) GAFFAR, MD. ABDUL The morphological character, genetic diversity, genotypic and phenotypic variance, genotypic and phenotypic coefficient of variation, heritability, genetic advance and genetic advance as percent of mean were studied for 15 genotypes of sponge gourd were determined in a field experiment conducted at the farm of Sher-e-Bangla Agricultural University, Dhaka during April, 2007 to October, 2007. Significant genotypic differences were observed for all the characters studied. The phenotypic coefficient of variation was higher than genotypic coefficient of variation in all the characters. The phenotypic coefficient of variation (FCV) estimates were high for all the characters. Heritability estimates were high for days to seed germination, days to first male flower, days to first female flower, peduncle length, fruit length and no. of seed per plant. In spite of high heritability values for most traits, the expected genetic advance as percentage of mean ranged from 1.14 to 121.30. Multivariate analysis was performed through principal component analysis (PCA); principal coordinate analysis, cluster analysis and canonical variate analysis were used to classify 15 sponge gourd genotypes. As per as PCA, 02 and cluster analysis, the genotypes were grouped into five different clusters. Cluster I and cluster IV had the maximum of four and minimum of one genotypes respectively. The highest inter-genotypic distance was found between 001 and 002 and the lowest distance between 002 and 007. The maximum inter-cluster distance was observed between the clusters IV and cluster V. whereas the lowest-inter cluster distance was found between the cluster I and cluster IV. The highest intra-cluster distance was identified in cluster Ill and the lowest intra cluster distance was found in cluster IV. Genotypes included in cluster I suitable for yield per plant, cluster Ill for having the highest mean value for inter node length, cluster V for leaf length, leaf breadth, petiole length, days to first male flower, days to first female flower, node no. of first male flower, node no. of first female flower and sex ratio. Considering diversity pattern and other agronomic performances the genotypic crosses between GOiand 002; 001 and GOT; 001 and 015: 001 and 004; 001 and 014; 001 and 010; Gil and G 07; 005 and 014: 002 and 003; 004 and Gil, might be used for future hybridization programme A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements For the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JANURAY-JUNE, 2008 Sun, 01 Jun 2008 00:00:00 GMT http://localhost:8080/handle/123456789/691 2008-06-01T00:00:00Z http://localhost:8080/handle/123456789/690 PLANT REGENERATION AND TRANSFORMATION IN KENAF (Hibiscus cannabinus) SHAMSUNNAHER Two different sets of experiments were conducted at the l3iotechnology Laboratory. Department of Genetic Resources and Seed Division. Bangladesh Jute Research Institute, Dhaka during the period of January 2007 to November 2007. In the first experiment, a detailed investigation was carried out to study the callus induction ability and subsequent plant regeneration protocol for two varieties of kenaf (I IC-2 and 1 C-95) using cotyledon with attached petioles as explants. In the second experiment. investigation was carried to study Açgiohc:ci rirun mediate genetic transformation. Two different investigations were made in the liNt experiment to slioss the effect of diflerent age of explants and BAP concentration in plant regenerati okenn oaff.. Five diliërcnt ages (6. 8. 10. 12 and 14 days) explants and seven different BAP concentrations were used and wide ranges of variation were observed. I lighest callus production (91.7%) and shoot regeneration (85.3%) 'cre found in I IC-2 when 10 days old explains were used. l3oth 1IC:-2 and I l('-95 showed the maximum callus initiation (91.7%) and the highest shoot regeneration (91.7%) in I lC-2 when MS medium supplemented with 3.0 mg/I BAP and 0.5 mg/I IAA was used. .1gmbacteriznn lumefuewis strain L8A440-1 was used Ibr transformation. containing selectable marker gene npIIl conferring resistance to kanamycin and GUS reporter gene also. Between the varieties. I IC-2 showed the highest response to GI.S expression (90.0% positive). Transgenic shoots produced roots and transferred to the soil successililly. A Thises Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JUNE, 2008 Sun, 01 Jun 2008 00:00:00 GMT http://localhost:8080/handle/123456789/690 2008-06-01T00:00:00Z