http://localhost:8080/handle/123456789/3366 2026-04-18T16:16:44Z http://localhost:8080/handle/123456789/3371 IMMUNO-STIMULATORY EFFECT OF LEVAMISOLE HYDRO CHLORIDE AND TRICLABENDAZOLE ON THE IMMUNE RESPONSE OF PESTE DES PETITS RUMINANTS (PPR) VACCINATION IN GOATS DHAR, NUPUR A Thesis Submitted to the Faculty of Animal Science and Veterinary Medicine Sher-e-Bangla Agricultural University, Dhaka-1207 In Partial Fulfilment of the Requirements For the degree of MASTER OF SCIENCE (M.S.) IN PARASITOLOGY DEPARTMENT OF MICROBIOLOGY AND PARASITOLOGY 2020-01-01T00:00:00Z http://localhost:8080/handle/123456789/3370 ISOLATION, IDENTIFICATION & MOLECULAR CHARACTERIZATION OF Escherichia coli AND Salmonella spp. FROM FECAL MATERIALS OF POPULAR PET BIRDS SEN, NIPU Recently, rearing of pet birds have increased with the increased socio-economic condition of the citizen. These pets population may contribute many zoonotic bacteria to public health. Salmonella spp. and E. coli are considered as the most common zoonoses in the world, causing important losses to public health. Considering these significances, this study assessed the occurrence of Salmonella spp. and Escherichia coli in caged Pigeon, Budgerigar & Lovebird. A total of 45 fecal samples were collected as environmental sample from these 3 different species of birds from pet shops of Katabon, Dhaka. Twenty samples from Pigeon (P-1 to P-20), Fifteen samples from Budgerigar (B-1 to B-15) & Ten samples from Lovebird (L-1 to L-10) were collected. Birds with positive cases of E. coli were found 22 (48.8%) & positive cases of Salmonella spp. were found 8 (17.7%). Rest 15 (33.33%) samples were unidentified. The occurrence of E. coli were 7 (35%), 11 (73.33%) and 4 (40%) in case of pigeon, budgerigar and lovebird respectively. The occurrence of Salmonella spp. in pigeon was 40%. No isolates of Salmonella spp. were found in budgerigar & lovebird. The antibiotic study revealed that all the isolates of E. coli were 100% sensitive to Ciprofloxacin, Norfloxacin, Amikacin, Azithromycin & Gentamicin. All the isolates of E. coli were 100% resistant to Tetracyclin, Ampicillin & Colistin sulphate. All the isolates of Salmonella spp. were 100% susceptible to Amoxicillin, Amikacin & Gentamicin and showed 100% resistance to Tetracycline, Trimethoprim & Colistin sulphate. These results analyze that birds that were analyzed may carry and spread these enterobacteria, and preventive measures for human exposure should be determined, as these microorganisms are public health concerns. A Thesis Submitted to the Department of Microbiology and Parasitology Sher-e-Bangla Agricultural University, Dhaka In Partial Fulfillment of the Requirements for the degree of MASTER OF SCIENCE (M.S.) IN MICROBIOLOGY 2020-01-01T00:00:00Z http://localhost:8080/handle/123456789/3369 A CROSS SECTIONAL INVESTIGATION ON THE OCCURRENCE OF TREMATODE METACERCARIAE IN FRESH WATER CRAB, Sartoriana spinigera IN BANGLADESH AL - WASEF Freshwater crabs are distributed throughout the tropical and sub-tropical regions of the world that are consumed for the purposes of medical and tonic properties, including the treatment of stomach ailments and physical injuries. Sartoriana spinigera is an important edible freshwater crab species which play an important role as intermediate host of Microphallus indicus. It serves as biological vector for transmission of their infective stages to the final host namely herring gulls, shorebirds, ducks and rodents. We targeted to investigate the prevalence of lung fluke in Bangladesh because Paragonimus westermani, the lung fluke has been reported from Northeast India including Assam, Meghalaya, Manipur, Arunachal. During survey, the metacercariae of food borne trematodes were investigated from a total of 261 crabs collected from different geographies of Bangladesh. We could not recover any metacercaria of Paragonimus spp. from the examined crab. The metacercariae of M. indicus were recovered from the crabs screened in this study. The overall prevalence of M. indicus metacercariae was 26% with intensity of 1-63 per crab. The highest (83.33%) prevalence was recorded from Naogaon district, northern part of Bangladesh followed by Sirajgonj (57.5%), Rajshahi (40%), Brahmanbaria (16%), Narsingdi (15%), Faridpur (10%), and Sunamgonj (7%). None of the crabs collected from Rangpur, Thakurgoan, Jashore, Meherpur, Magura, Barguna, Sylhet, Kishorgonj, Cox’sBazar and Bandarban was found to be infected with any microphallid metacearia. Whereas, the highest number of infected crabs were found the in rivers (56%), followed by haor, canal and ditches (5%) and ponds (0%). To the best our knowledge, M. indicus is going to be reported for the first time from Bangladesh. Therefore, proper attention should be paid during consumption of crabs. A Thesis Submitted to the Department of Microbiology and Parasitology Sher-e-Bangla Agricultural University, Dhaka In Partial Fulfillment of the Requirements for the degree of MASTER OF SCIENCE (M.S.) IN PARASITOLOGY 2020-01-01T00:00:00Z http://localhost:8080/handle/123456789/3368 ISOLATION, IDENTIFICATION & ANTIBIOGRAM OF BACTERIA ASSOCIATED WITH CAT ORAL CAVITY: A NEGLECTED ISSUE AHMED, SHAMIMA This research work was conducted to isolate, identify and antibiotic sensitivity profiling of bacteria found in oral cavity of pet cat in Dhaka city. A total number of 40 samples were collected aseptically from Central Veterinary Hospital (CVH), Dhaka and transported to National Institute of Biotechnology laboratory, Savar. Total viable count (TVC) of bacteria from all samples (n = 40) was determined. Hundred percent prevalence of organisms was noted with the highest TVC, 1.199×10 ii 13 and the lowest TVC, 3.60×10 2 . The isolation and identification of bacterial genera/species were performed by cultural characterist ics, Gram’s staining, biochemical tests and molecular identification to some extent. The prevalence of Escherichia coli, Salmonella spp., Staphylococcus aureus and Staphylococcus epidermidis were 100%, 5 %, 57.5% and 7.5 % respectively. Both pathogenic and non-pathogenic E. coli were confirmed by PCR and DNA sequencing. Antibiotic sensitivity test by disc diffusion method was performed against seven different antibiotics. E. coli isolates showed the highest sensitive to gentamycin (80%) followed by azithromycin (70%) and the highest resistant to ampicillin. Isolates of Salmonella isolates were found to be highest sensitive against gentamycin (100%) followed by azithromycin (50%). Highest resistant pattern of Salmonella spp. was showed against ampicillin (100%). Salmonella spp. showed 50% resistant to erythromycin, streptomycin and tetracycline. S. aureus showed the highest sensitivity to gentamycin (80%), followed by cotrimoxazole (60%) and the highest resistance pattern was shown against ampicillin (100%), followed by erythromycin (80%) and tetracycline (60%). S. epidermidis showed the highest (100%) resistant against ampicillin and the highest (100%) sensitive to gentamycin. The findings from current study recommend that pet cats in Dhaka city contain multi-drug resistant E.coli, Salmonella spp., S. aureus. Only S. epidermidis was not found as multidrug resistant. This multi-drug resistant phenomenon can cause a potential public health hazard through transmission to humans by direct contact or the food chain or the evolved way of life. A Thesis Submitted to the Faculty of Animal Science and Veterinary Medicine Sher-e-Bangla Agricultural University, Dhaka-1207, In Partial Fulfillment of the Requirements for the degree of MASTER OF SCIENCE (MS) IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY AND PARASITOLOGY 2020-01-01T00:00:00Z