http://localhost:8080/handle/123456789/3099 2026-04-18T16:15:28Z http://localhost:8080/handle/123456789/3475 IN-VITRO EVALUATION OF SEED TREATMENT BY COMMERCIAL FORMULATIONS OF TRICHODERMA ON SEEDLING QUALITY AND DROUGHT RESPONSES OF RICE AKTER, SUBORNA A pot experiment was conducted in Net House at Department of Plant Pathology, Shere-Bangla Agricultural University, Dhaka-1207, Bangladesh during the period from January 2018 to April 2018 to evaluate seed treatment by different commercial Trichoderma formulations seedling quality and drought responses of rice following CRD with five replications. Two drought tolerant varieties BRRI dhan 56, 71 and one drought susceptible check variety IR 64 and six treatments viz. T = G-derma (powder), T 2 = Bioderma, T 3 = Recharge, T 4 = Decoprima, T ii 5 1 = G-derma (LDS) and T = Control were used in the experiment. Soil was sterilized by 2% formalin solutions. Seed related data was recorded on % seed germination, speed of germination (Germination Index), mean germination time (MGT), mean daily germination (MDG), peak value (PV) and germination value (GV). Moreover, data was recorded on seedling growth characteristics viz. number of leaves, number of tillers, plant height, shoot length, root length, fresh shoot and root weight, dry shoot and root weight, seedling vigor index (SVI) and seedling vegetative vigor (SVV) at different days after sowing. At 35 DAS, drought treatments were given and drought response data viz. droopy leaf, rolled leaf, drying leaf and drought sensitive scale was recorded at 4, 7, 10 and 13 days drought stress (DDS). The data were varied significantly among the treatments. Considering seed germinations parameters, T (Decoprima) showed the best results followed by Gderma (powder) and Bioderma. However, in case of seedling growth, (Decoprima) showed the highest performance followed by G-derma (LDS) and Recharge. Moreover, the lowest drought responses were observed in Decoprima treated seeds followed by Gderma (LDS) and Recharge. The findings revealed that, seed treatment with Trichoderma can mitigate short duration drought stress in rice plant. No disease was observed in rice seedlings due to sterilization of soils and treatments of seeds. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, In Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN PLANT PATHOLOGY 2018-01-01T00:00:00Z http://localhost:8080/handle/123456789/3474 STANDARDIZATION OF HOT WATER TREATMENT IN CONTROLLING MAJOR SEED BORNE PATHOGENS OF VEGETABLES SULTANA, NASRIN The experiment was conducted to standardize the hot water in controlling major seed borne pathogens of selected vegetables during the period from September 2017 to March 2018, in the central laboratory of the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207. Effect of dipping seed in hot water at 50 to 60 °C for 5 minutes against seed borne microflora were explored to standardize the temperature and time in controlling seed borne pathogens of bitter gourd, pumpkin, bottle gourd, cucumber, eggplant, chilli, tomato, lady´s fingers, radish and country bean seeds. The treatments were T 0 T 3 T 6 = Control, T 1 = 54 ºC for 5 minutes, T 4 = 50 ºC for 5 minutes, T 2 =56 ºC for 5 minutes, T 5 = 52 ºC for 5 minutes, = 58 ºC for 5 minutes and = 60 ºC for 5 minutes. Data were collected on % seed germination, % seed infection, % rotten seed and % dead seed. The experiments were conducted following Completely Randomized Design (CRD) and analyzed by computer package MSTAT-C. Significant differences were found in all the parameters. The highest seed germination in all the vegetables were recorded at the treatment where seeds were treated at 54 °C to 58°C for 5 minutes and the lowest were obtained at control. The lowest rotten seed of all the vegetables recorded in 60 °C and the highest rotten seeds obtained at T 0 (control). The highest dead seed of studied vegetables were recorded in T 6 (60 °C for 5 minutes) and the lowest were obtained at T 0 (control). The lowest infected seed of studied vegetables were recorded in treatment T 6 (60 °C for 5 minutes) and the highest infected seeds were obtained at T 0 (control). In most cases Chaetomium sp., Aspergillus spp., Penicillium spp., and Fusarium spp. etc were prevalent in the seed samples. So, hot water seed treatment with 54 °C to 58°C for 5 minutes could be suggested as safe, pollution free and environmental friendly for management of seed borne pathogens of selected vegetables. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, In Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN PLANT PATHOLOGY 2018-01-01T00:00:00Z http://localhost:8080/handle/123456789/3473 SCREENING OF SELECTED GARLIC VARIETIES AGAINST FUSARIUM ROT DISEASE CAUSED BY Fusarium proliferatum PRINKY, JANNATUN NAHAR A field experiments was carried out to study the screening of selected garlic varieties in field and isolation, identification and pathogenicity test of Fusarium rot caused by Fusarium proliferatum was also done. The experiment was conducted in central farm and plant pathology lab, Sher-e-Bangla Agriculture university, Dhaka-1207 with RCBD (Randomized Complete Block Design) with three replications. The entire experimental plot was divided into three blocks, each of which then divided into 24-unit plots. A total eight varieties viz. BAU Rashun-1, BAU Rashun-2, BARI Rashun-1, BARI Rashun-2, BARI Rashun-3, BARI Rashun-4, Local Deshi and Local Indian was selected to conduct the study. In the following study the highest pre emergence mortality was observed in BAU Rashun-1 garlic variety with 31.35% germination failure and the lowest were found in BARI Rashu-1 with 14.19%. The maximum result was found for the post emergence mortality in BARI Rashun-3 with 13.22% mortality rate after germination and minimum result were found in Local Indian variety 6.15%. Leaf height showed negative correlation with the entire yield parameters considered and become significant at 0.01% level of probability and number of leaves showed positive correlation with all the yield defining characters except clove diameter (-.859). For disease incidence BARI Rashun-4 showed the highest susceptibility to Fusarium rot and maximum disease severity of garlic variety was observed in BARI Rashun-4. Maximum radial mycelial growth of Fusarium proliferatum was observed in BAR3I (9mm). The minimum radial mycelial growth was BAR2I (5.2mm). Pathogenicity test was done with only one susceptible variety BARI Rashun-4 in in-vivo condition by inoculating Fusarium proliferatum as soil inoculation. 27 pots were considered as test pot, 29.62% pre emergence mortality and 49.37% dead seedling was observed in in- vivo condition. A Thesis Submitted to the Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2018-01-01T00:00:00Z http://localhost:8080/handle/123456789/3472 INCIDENCE OF COCONUT DISEASES IN SELECTED AREA OF BANGLADESH AND STUDY OF BIOCONTROL BASED MANAGEMENT PACKAGE AGAINST BASAL STEM ROT OF COCONUT RAHMAN, SUMONA A survey study and lab experiment was conducted in this study. The survey study was held on four district. Three are coastal area viz. Noakhali, Barisal and Patuakhali and another is in SAU campus in Dhaka. The lab experiment was conducted in Molecular Biology and Plant Virology Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, during January 2018 to March 2019. The study was aimed to demonstrate occurrence and incidence of basal stem rot and foliar diseases of coconut in surveyed area and to develop a biocontrol-based management package against basal stem rot. The disease incidence of basal stem rot was measured 65%, 64.44%, 60.83%, 49.17%, in SAU, Patuakhali Noakhali and Barisal respectively. The disease severity was 33.88%, 30%, 26.67%, and 24.58% respectively in Noakhali, SAU, Patuakhali and Barisal. The incidence of foliar diseases was estimated 85%, 85%, 78.61%, and 77.78% respectively in Noakhali, SAU, Patuakhali and Barisal. The disease severity was 39.13%, 33.56%, 28.33% and 24.13% respectively in Noakhali, Patuakhali, SAU and Barisal. The highest incidence of basal stem rot (27.5%) and leaf disease (19.2%) was found in loamy soil. The highest disease incidence was found at the soil pH 6-7 and the incidence were 26.75% and 22.45 respectively. The highest incidence of basal stem rot (24%) and foliar diseases (18%) was found in plant age group 36-40 years. In total 120 root samples and 90 leaf samples were collected for aseptic isolation. The isolated pathogens from root samples were identified as Ganoderma sp. while three pathogens were isolated from leaf samples which are Pestalotia sp., Curvularia sp., and Alternaria sp. In total 32 Ganoderma infected plants from SAU campus were selected and treated with 3 management package of different bioagent and botanicals viz., Trichoderma viridae, cattle urine, masturd oil cake, neemseed extract and garlic extract. After 5 months of treatment root samples were collected and investigated for existence of pathogen .Among the three combinations mustard oil cake + neemseed extract + Trichoderma viridae and cattle urine gave the best performance and disease inhibition were 35% and 52.5% over control. The result of the present study revealed that basal stem rot and foliar diseases are frequently occurred in the survey areas and cattle urine was found effective in the management of this disease in coconut. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN PLANT PATHOLOGY 2018-01-01T00:00:00Z