http://localhost:8080/handle/123456789/2906 2026-04-18T16:15:31Z http://localhost:8080/handle/123456789/3162 MOLECULAR DIVERSITY STUDY OF DIFFERENT HYBRID VARIETIES OF BOTTLE GOURD (Lagenaria siceraria Mol.) MUNNI, MAHBUBA HOQUE Bottle gourd (Lagenaria siceraria Mol.) is one of the most important Cucurbitaceous vegetables in the world. It is widely grown for leafy and green fleshy vegetables. This study was conducted to determine the molecular diversity among eight bottle gourd hybrid varieties which were collected from three renowned private Seed Companies (Metal Agro Limited, ACI Limited, Lal Teer Seed Limited) of Bangladesh. A total of nine primers (RAPD, ISSR and SSR) were used to determine polymorphism among bottle gourd hybrids to produce scorable DNA bands. Total 26 bands were amplified by nine primers. Among them 18 were polymorphic bands. The range of DNA amplification varied from 1000-100 bp. Average 64.82% polymorphism was obtained among the genotypes. Genetic diversity ranged from 0.2188 to 0.5000 and the frequency of major allele ranged from 0.5000 to 0.8750. Nei’s genetic distance ranged from 0.2308 to 0.7308. The PIC value ranged from 0.1948 to 0.3750 with an average value 0.3243. The PIC value indicates that the studied bottle gourd hybrids had medium level of molecular diversity. A dendrogram indicating the relative genetic similarity of the bottle gourd hybrids was constructed which followed three major clusters (A, B and C) among the studied material. The two hybrid H7 and H8 showed more similarity in genetic identity that fall in the same group-B which has collected from same seed company. Likewise, the hybrid H5 and H6 classified in the same group-C were also obtained from same seed company. Group-A contains the two hybrids H3 and H4 which are collected from Lal Teer Seed Ltd. The present investigation revealed that, the bottle gourd hybrid developed by the seed companies has more similarity within their own genotypes but to some extend genet ically diverse between different seed companies. These experimental findings can be used for the protection of hybrids in commercial purpose and in managing bottle gourd genetic resource in Bangladesh. A Thesis submitted to The Department of Biotechnology, Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka-1207 In partial fulfilment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY SEMESTER: JULY- DECEMBER, 2018 2018-12-01T00:00:00Z http://localhost:8080/handle/123456789/3161 MICROPROPAGATION OF STRAWBERRY (Fragaria×ananassa Duch.) ISLAM, MD. MONIRUL The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla Nagar, Dhaka-1207 from the period of May 2018 to November 2018 for in vitro renegaration of strawberry. The healthy, disease free shoot tips of 0.501.0 cm length were used as explants, It was sterilized by 0.1% HgCl 2 mixing with few drops Tween-20. The explant was inoculated in MS media supplemented with different combination of the BA (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) and IBA (0.50, 1.00, 1.50, 2.00 and 2.50 mg/l) either alone or in combination. The maximum days for shoot induction were recorded in control treatment. The highest number of shoots (3.60) in BA 2.0 mg/l and leaves (9.60) per explant observed in BA 3.0mg/l. In contrast combine treatment of 2.0 mg/l BA+1.00 mg/l IBA performed best in case of percentage shoot induction (80%) and number of shoots (3.60).The highest length of shoots (3.66 cm) in BA 2.0 mg/l+ IBA 2.0mg/l . It was noticed that the minimum days to shoot induction, percent shoot initiation and no. of shoot were recorded in 2.0 mg/l BA+1.0 mg/l IBA. The highest number of leaves per explant was recorded in 2.0 mg/l BA+0.5 mg/l IBA. The highest number of root was observed in 2.0 mg/l BA+ 1.5 mg/l IBA and the highest percent root initiation in 3.0 mg/l BA+1.5 mg/l IBA Overall, micropropagation was found to be very effective and promising method in the proliferation of strawberry and this experiment can be a useful tool for breeding program of strawberry. Finally, the survival rate of micropropagated plantlets were observed in open atmosphere 75% and 80% in controlled condition. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE IN BIOTCHNOLOGY SEMESTER: JUNE-JULY, 2018 2018-12-01T00:00:00Z http://localhost:8080/handle/123456789/2953 IN VITRO PROPAGATION OF TURMERIC (Curcuma longa L.) ANIK, SAMIUL HOSAIN The present study was undertaken in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka1207, during the period from February 2018 to June 2018 to evaluate the effect of different plant growth regulators (BA, IBA) on in vitro plantlet regeneration of Turmeric. The healthy, disease free sprouts of turmeric were used as explants which were inoculated in MS media supplemented with different conc. of Benzyl adenine (BA) (1.0, 2.0, 3.0 and 4.0 mg/L) and IBA (1.0, 2.0, 3.0 and 4.0 mg/L) either alone or in combination for shoot and root regeneration. The experiments were arranged in Randomized Completely Block Design (RCBD) with three replications. The maximum shoot induction was obtained with 2.0 mg/L BA. The highest shoot length (5.93 cm) was noticed in BA 2.0 mg/L + IBA 3.0 mg/L and minimum shoot length (1.4 cm) with control MS dose was observed. The highest percentage (81.00%) of shoot induction was recorded with BA 2.0 mg/L in minimum (14.0 days). Where, IBA 3.0 mg/L had produced the highest percentage of root induction (82.00%) required minimum (20 days). The highest length of shoot (3.4 cm, 5 cm and 5.9 cm) at 21, 28 and 42 DAI respectively was found in BA 2.0 mg/L + IBA 3.0 mg/L. The treatment BA 2.0 mg/L + IBA 1.0 mg/L gave the highest number of root (6.0, 8.6, 12.67) at 21, 28 and 42 DAI respectively and the highest length of root (2.7 cm, 4.8 cm and 5.4 cm) at 21, 28 and 42 DAI, respectively was found in BA 2.0 mg/L + IBA 1.0 mg/L. Regenerated plantlets showed, 100% survival during in growth chamber conditions and 86.66% in shade house stage of hardening and 83% in open atmosphere at direct sunlight. Therefore, an efficient protocol has been developed for in vitro propagation of Turmeric which has great commercial value for year round production in Bangladesh. A Thesis Submitted to the Department of Biotechnology, Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka-1207, In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY SEMESTER: JANUARY- JUNE, 2018 2018-06-01T00:00:00Z http://localhost:8080/handle/123456789/2917 POLYMORPHISM STUDY AND MOLECULAR DIVERSITY ANALYSIS OF DIFFERENT HYBRID VARIETIES OF RICE (Oryza sativa L.) THROUGH RAPD AND SSR MARKERS ISLAM, ABSANA Rice (Oryza sativa L.) is one of the most important cereal crop around the world. It is the staple food in Bangladesh. This study was conducted in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207 from the period of November 2018 to April 2019 to determine the molecular diversity among four rice hybrid varieties using Random Amplified Polymorphic DNA (RAPD) and Single Sequence Repeat (SSR) markers. A total of 21 RAPD primers and 4 SSR primers were used to determine polymorphism among the rice hybrids to produce scorable DNA bands. Total 82 bands were amplified through RAPD primers. Among them 26 were polymorphic bands. The range of DNA amplification varied from 1300-150 bp. The rate of polymorphism was obtained about 30.33%. Lower level of polymorphisms in hybrids indicated that there is a basic similarity among the varieties which is due to the common ancestor and the similarity of the selective traits. Genetic diversity ranged from 0 to 0.5000 and the frequency of major allele ranged from 0.5000 to 1.0000. Nei’s genetic distance ranged from 0.1190 to 0.2262. The PIC value ranged from 0 to 0.3750 with the average value 0.1052. The PIC value indicates that the studied rice hybrids had low molecular diversity. A dendogram indicating the relative genetic similarity of the rice hybrids was constructed which followed three major clusters (A, B and C) among the studied material. Two SSR primers showed polymorphic band which can be used as an evidence of variety protection data. This experimental findings can be used for the protection of hybrids in commercial purpose and in managing rice genetic resource in Bangladesh. A Thesis Submitted to The Department of Biotechnology, Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka-1207 In partial fulfillment of the requirements For the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY SEMESTER: JANUARY-JUNE, 2018 2018-06-01T00:00:00Z