http://localhost:8080/handle/123456789/2819 2026-04-18T16:16:40Z http://localhost:8080/handle/123456789/2286 MOLECULAR DIVERSITY ANALYSIS AND POLYMORPHISM STUDY IN COTTON (Gossypium hirsutam L.) GENOTYPES THROUGH RAPD MARKERS WAHAB, MD. ABDUL The experiment was conducted at the Laboratory of the Department of Biotechnology, Sher-eBangla Agricultural University (SAU), Dhaka- 1207, Bangladesh. The period of the experiment was March 2018 to November 2018. Cotton (Gossypium hirsutum L.) is the leading fiber crop and the second most important oil seed crop in the world. This study aimed to analyze the molecular diversity among 9 cotton genotypes collected from Cotton Development Board, Bangladesh using the Random Amplified Polymorphic DNA primer. A total of 11 RAPD primers were used to determine polymorphism among the genotypes and finally 6 of the primers produced scorable DNA bands. Some total of with 30 DNA bands were obtained and among them 9 were polymorphic bands. The ranged of DNA amplification varied from 160 to 1500 bp. The rate of polymorphism was calculated about 31.57%. Genetic diversity ranged from 0.22 to 0.44 with an average value of 0.30. Nei’s genetic distance ranging from 0.1667 to 0.6667 and most importantly PIC value ranged from 0.18 to 0.35 with an average value of 0.27. The PIC value indicates that most of the studied cotton genotypes was moderately diversified and homogenous as well as no heterozygosity found. A dendogram indicating the relative genetic similarity of the Bangladeshi cotton genotypes was constructed which followed in two major cluster (A and B) among the studied material. The results also showed that the genotypes can be separated from each other at the molecular level by taking advantage of some of the RAPD markers. The Cotton Development Board, Bangladesh committed to work on cotton improvement and so for this was the first time in CDB to investigate molecular diversity and DNA profiling in cotton using CDB collected cotton genotypes. This experiment can be used as a baseline for the future molecular research work on cotton genotypes in Bangladesh. DEPARTMENT OF BIOTECHNOLOGY SHER-E-BANGLA AGRICULTURAL UNIVERSITY DHAKA-1207 2017-01-01T00:00:00Z http://localhost:8080/handle/123456789/2285 DNA MARKER BASED HYBRIDITY TEST OF DIFFERENT EGGPLANT (Solanum melongena L.) HYBRIDS HOSSAIN, MAHBUBA An experiment was carried out to identify the hybrid variety of eggplant through SSR marker. Four eggplant hybrids vizMEH5003, MEH5006, MEH5009, MEH5012 and their corresponding parents were used as experimental materials. The hybrid varieties of eggplant were developed by Metal Seed Ltd., Dhaka, Bangladesh. The experiment was conducted at the Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Initially different SSR primerswere used to establish polymorphism among the parents of each hybrid. The identified polymorphic primers were used to screen the hybrid variety together with corresponding parents. It was noticed that only one primer viz MEH SSR-04 showed polymorphism in the parents of MEH5009 hybrid. This primer was used for the detection of hybrid variety. A 150bp DNA fragment was amplified in male parent and hybrid variety which was absent in female genotype. This DNA band can be used for large scale screening of that specific hybrid variety for seed purity in commercial purpose. The parents of other hybrid showed monomorphic DNA band and some of the SSR primerswere not amplified in any eggplant genotype under investigation. The confirmation of genetic purity in seeds is very important for identification of genetic contamination and hybrid purity for farmers and plant breeders before sale of the seed to public domain. The genetic purity test by conventional method called Grow Out Test DEPARTMENT OF BIOTECHNOLOGY SHER-E-BANGLA AGRICULTURAL UNIVERSITY DHAKA-1207 2017-01-01T00:00:00Z http://localhost:8080/handle/123456789/2040 STUDY ON POTATO (Solanum tuberosum L.) REGENERATION CAPABILITY IN AMMONIUM NITRATE FREE MEDIUM COMPOSITION HENA, HASNA The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period of January, 2016 to June, 2017 to evaluate the performance of ammonium nitrate free medium for in vitro regeneration of a potato (Solanum tuberosum L.) variety, Asterix. Sprouts and nodal segments were used as explants. A new chemical which is indicated as β chemical had been used in this research. Five different compositions of the stock solution-01 of the MS medium were used which were marked as Treatment-01 (standard MS dose, 1962), Treatment-02 (NH 4 free stock solution), Treatment-03 (NH 4 NO 3 free stock solution with double dose of other ingredients), Treatment-04 (stock solution-01, developed in the Department of Biotechnology, SAU, Dhaka ) and Treatment-05 (readymade MS powder). In vitro regeneration of a potato was successfully achieved in those media. The parameters like shoot length, shoot number, shoot diameter, node number per plantlet, leaf number per plantlet were highest in Treatment-04 at 14, 21 and 28 days of regeneration. But in case of root number and root length (cm) per plantlet, Treatment-05 showed the highest result and in some cases it was statistically similar with Treatment-04. Treatment-02 showed lowest result in each of the said parameter. It might be due to the low concentration of nitrogen and it indicated that NH 4 x NO 3 is essential for in vitro regeneration of potato. Treatment-03 performed moderately in most of the parameters. Asterix variety showed significant variation in case of Treatment-01 and Treatment-05 on most of the parameters under studied. Therefore, a relliable medium composition for in vitro potato regeneration has been established. So, the stock solution-01 which was developed from the Department of Biotechnology, SAU, Dhaka can be the best alternative to NH 4 in tissue culture study of plants. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY 2017-01-01T00:00:00Z http://localhost:8080/handle/123456789/2039 IN VITRO REGENERATION OF ONION (Allium cepa L.) UNDER SALT STRESS CONDITION PLABON, MD. ASFIQUR RAHMAN The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla Nagar, Dhaka-1207 from the period of September 2016 to July 2017 for in vitro regeneration of onion (Allium cepa L.) under salt stress condition. Three genotypes namely Faridpuri, Taherpuri and Pusa red (Indian) were used as investigated genotypes in this study. Shoot tip segments of these genotypes were cultured in MS (Murashige and skoog, 1962) media having 0.0 (control), 25, 50, 75 and 100 mM NaCl. The shoot tips of experimental onion genotypes were not significantly affected upto 75 mM salinity level in comparison with control treatment. The experiment was conducted at two factorial (variety and Treatment) Completely Randomized Design (CRD) with 3 replications for each treatment. Salinity response on days required for shoot regeneration varied significantly from different salinity condition. The genotype Faridpuri was found maximum tolerant upto 100mM salinity level with 10.60 cm shoot length and 1.43 cm root length having the highest relative shoot and root growth. Besides, Pusa red was found as salinity sensitive genotype showing the lowest 7.03 cm shoot length and 0.60 cm root length at 100 mM NaCl treatment. However, Taherpuri was found tolerant up to 75 mM salinity level with 8.30 cm shoot length and 1.43 cm root length, respectively. The genotypes Faridpuri, Taherpuri and Pusa red (Indian) can be used for further investigation in field condition to evaluate their performance at various salinity levels. Therefore, a convenient in vitro regeneration protocol of onion genotypes under different salinity level has been developed which can be used for screening salinity tolerant variety of onion within a very short period of time. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY 2017-01-01T00:00:00Z