http://localhost:8080/handle/123456789/2818 2026-04-18T17:38:45Z http://localhost:8080/handle/123456789/414 IN VITRO PROPAGATION OF GARLIC (Allium sativum L.) MUBARRAT, FABEEHA The research work was conducted in the Laboratory of Biotechnology of the Department of Biotechnology, Sher-e-Bangla Agricultural University, from the period of July 2015 to June, 2016 to observe the response of different plant growth regulators on in vitro regeneration of garlic. The disease free, healthy and sterilized basal part of clove of 2-3 cm length were used as explants. Sterilization was done with 70% ethanol, 0.5% HgCl 2 along with few drops of Tween-20. Explants were placed in MS medium supplemented with the Kinetin (KIN), alone and in combination with 2, 4-Dichlorophenoxy acetic acid (2, 4-D). The highest shoot regeneration percentage (100%) was observed by 3.0 mg/L of KIN alone and by wide range of KIN in combination with 2, 4-D (i.e. all combination of 2.5, 3.0 mg/L KIN with 1.0, 1.5, 2.0, 2.5 mg/L 2, 4-D) . The highest shoot length (34.50 cm) was observed after 3 weeks of initiation in 3.0 mg/L KIN treatment. Combined doses of KIN and 2, 4-D showed 1 shoot after 3 weeks of initiation. Number of leaves did not exceed 3 and it was noticed through all combination of 2.0, 2.5, 3.0 mg/L KIN with 1.0, 1.5, 2.0, 2.5 mg/L of 2, 4-D. The highest root induction (100%) was observed by 2.5 mg/L KIN + (1.0, 1.5) mg/L 2, 4-D. It took minimum 3 days to induce root. The average and the highest length of root (3.5 cm and 2.93 cm respectively) was noticed by application of 3 mg/L KIN and 1.0 mg/L 2, 4-D. Maximum number of roots (10.67) were developed by 3.0 mg/L KIN+ 1.5 mg/L 2, 4-D. After transferring the plantlets in the field condition, 86% survival plants were recorded. Finally, in vitro regeneration of garlic was proved to be less time consuming. Moderate doses of hormones showed satisfactory responses in shoot proliferation and root induction which made the tool cost effective as well. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY SEMESTER: JANUARY-JUNE, 2016 2016-12-01T00:00:00Z http://localhost:8080/handle/123456789/413 PHENOTYPIC CHARACTERIZATION AND GENETIC DIVERSITY ANALYSIS OF EGGPLANT (Solanum spp.) GERMPLASM USING SSR MARKER TASRIN, KASHPIA An experiment was carried out with twenty five local and two wild relatives (Solanum sisymbriifolium and S. torvum) of eggplant at the research field and in the Biotechnology Laboratory of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh, during October 2015 to June 2016 to investigate the phenotypic characterization and molecular diversity analysis of different local eggplant germplasm and its wild relatives. The morphological study revealed diversified characters among all the genotypes. Variations were observed in color of leaf vein, stem as well as fruit size and shape. Maximum yield per plant was recorded in Salta Begun and it was 9.8 kg. The minimum yield per plant was observed in both the wild species (3.8 kg) as they produced very small fruits. Correlation of yield contributing traits separated all the genotypes into four clusters (A, B, C, and D). Among them cluster D had five genotypes together with the wild species indicated their relatedness. Five well-known SSR primers were used for the molecular characterization of the genotypes. Ten alleles, ranged from 1 to 3 alleles per locus and an average of 2.0 were detected. Among five primers three were able to produce polymorphic bands and the total number of polymorphic bands was five. The highest (2) number of bands was observed in SSR primers EPSSR82 and smSSR01. The Polymorphism Information Content (PIC) of SSR markers ranged from 0.37 (smSSR01) to 0.67 (EPSSRR82) with an average value of PIC = 0.54. Gene diversity ranges from 0.49 (smSSR01) to 0.72 (EPSSR82), with an average value of 0.61. The value of pair-wise comparisons of Nei‟s (1972) genetic distance (D) and identity between varieties was computed from combined data for the five primers, ranged from 0.20 to 1.00, with an average value of 0.60. Multivariate cluster analysis on the basis of Nei‟s genet ic distance and identity revealed that within those 27 genotypes there surely do exist considerable diversity. UPGMA method separated the segregation of 27 genotypes into two major clusters (I and II). From the clusters, wild species Solanum torvum belonged to the sub-cluster (IIb), that revealed its distinct variation from the others. On the other hand, wild species Solanum sisymbriifolium showed a close relatedness by forming the same cluster together with thirteen local eggplant genotypes. This experiment brought a great opportunity for eggplant improvement as the local genotypes had a close relation with the wild species. A Thesis Submitted to The Department of Biotechnology, Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka-1207 In partial fulfillment of the requirements For the degree Of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY SEMESTER: JANUARY-JUNE, 2016 2016-12-01T00:00:00Z http://localhost:8080/handle/123456789/412 RAPID GENOMIC DNA EXTRACTION IN BULB CROPS AND ESTIMATION OF GENETIC DIVERSITY IN GARLIC (Allium sativumL.) USING RAPD MARKER SHEULY, KAMRUN NAHAR An experiment was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University (SAU), Dhaka, Bangladesh to develop low cost and rapid genomic DNA isolation technique and to study the DNA fingerprinting and genetic diversity of nine garlic genotypes. Conventional CTAB/SDS method of DNA extraction required more time and cost. In contrast, the newly developed method to low cost and very fast method for extraction of genomic DNA in bulb crops. The garlic germplasms were BARI-G1, GC001, GC008, GC0012, GC0013, GC0017, GC0024, GC0027 and GC0028. Genomic DNA was extracted in both methods to perform PCR reaction with seven RAPD primers. Sum of 53 distinct and differential amplified DNA bands were generated from PCR amplification. Out of them, 40 bands (75.88%) were polymorphic and 13 bands (24.12%) were monomorphic. The Nei’s genetic identity among 9 garlic genotypes ranged from 0.8053 to 0.9966 and genetic distance ranged from 0.0053 to 0.2166. The UPGMA Dendrogram segregated the 9 garlic genotypes into two main clusters. The first cluster contained 1 genotypes and the second cluster had 8 genotypes. The Dendrogram also indicated that BARI-G1 vs GC0027 varietal pair showed highest Nei’s genetic distance (0.2166) and GC0012 vs GC0017 varietal pair showed lowest genetic distance (0.0053). The study revealed a significant amount of relationship and genetic diversity among the studied 9 garlic genotypes. The RAPD markers were found to be useful tool for molecular characterization and polymorphism study in garlic genotypes. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka In partial fulfillment of the requirements For the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY SEMESTER: January - June, 2016 2016-12-01T00:00:00Z http://localhost:8080/handle/123456789/411 MICROPROPAGATION OF SALT TOLERANT EXOTIC POTATO GENOTYPES AND THEIR IN VITRO BIOASSAY RAHMAN, MD. HABIBUR In vitro bioassay of nine exotic potato genotypes namely CIP102, CIP106, CIP111, CIP 117, CIP 120, CIP124, CIP127, CIP 136 and CIP 139 was conducted for salinity tolerance at Tissue Culture Lab, Tuber Crops Research Center (TCRC), Bangladesh Agricultural Research Institute (BARI), Gazipur-1701. Single node and root tip segments of these genotypes were cultured in MS media supplemented with 0.0 (control), 80, 100, 120, 140 and 160 mM NaCl. In case of in vitro shoot bioassay, CIP 139 was found as the most salt tolerant with the highest plant height (9.67 cm), number of nodes (9.50), number of leaves (13.60), number of roots (8.00), length of root (6.50 cm), fresh weight of shoot (509.0 mg) and fresh weight of root (205.60 mg) at 160 mM NaCl (14.61 dSm ). On the other hand, CIP 106 was found as the most salinity sensitive at 120 mM NaCl (10.96 dSm -1 ) producing minimum plant height (7.17 cm), number of nodes (6.50), number of leaves (12.50), number of roots (9.70), length of roots (5.10 cm), fresh weight of shoot (572.3 mg) and fresh weight of root (244.4 mg) followed by CIP 136, CIP 117 and CIP 111 at same salinity level. However, CIP 127, CIP 102 and CIP 124 genotypes showed very good performance up to 140 mM NaCl (12.78 dSm -1 -1 ). In vitro root bioassay also revealed the highest salinity tolerance of CIP 139 up to 160 mM NaCl, CIP 127 and CIP 102 up to 140 mM NaCl as well as CIP 106 up to 120 mM salinity level. The root tips of experimented potato genotypes were not significantly affected up to 120 mM salinity level in comparison with control where CIP 120 was found as the lowest tolerant up to 120 mM salinity level in MS media. Among the 0.0 (control), 0.1, 0.5, 1.0 and 1.5 mg /L IBA concentrations supplemented with MS media 1.0 mg/ L was found best for rooting with early root initiation (6.57 days) and well development (10.48 days), highest root length (8.63 cm), 2 highest root number (17.25) and maximum fresh weight of root (332.22 mg). Interestingly, there was no significant differences between 0.5 and 1.0 mg/L IBA for in vitro root induction and development in the experimented CIP potato genotypes. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY Semester: January-June 2016 2016-12-01T00:00:00Z