http://localhost:8080/handle/123456789/2724 2026-04-18T16:17:06Z http://localhost:8080/handle/123456789/4321 ROOT COLONIZATION AND PERSISTENCE· OF Purpureocillium lilacinum IN RBIZOSPHERE AS INFLUENCED BY SOME CROP SPECIES AND Meloidogyne incognita RAHMAN, MD. MOSTAQUR The 'effect of ten crop species along with two different rooting media viz" coco dust and soil on the root colonization ability of the fungal BCA Purpureocillium lilacinum and its persistence in rhizosphere of the ten crop species along with the presence or absence of nematode were evaluated through a test tube experiment in laboratory and, a pot experiment in shade house. P. lilacinum resulted, in root colonization in all of the crop species in varying percentage depending on the rooting media and the crop species themselves. A constant 100% and an average of 45.30% root colonization were obtained in coco dust and in soil, respectively. In soil, a maximum and a minimum root colonization was observed in cucumber (67.17%) and chickpea (30.55%), respectively; whereas maize (55.50%), potato (50%), brinjal (48.14%), cabbage (44.28%), rice (44.26%) and tomato (43.45%); wheat (37%), soybean (32.07%) gave statistically similar result to cucumber and chickpea, respectively. Population dynamics of the fungus showed no significant difference between soil without crop species and soil from the root zone of majority of the test crop species. Overall, 8 out of 10 crop species showed higher densities compared to soil. Of them, 3 (maize, brinjal, soybean) showed significantly higher CFU/g of soil whereas the-rest 5 (rice; wheat, potato; cucumber, chickpea) showed insignificantly higher CFU/g of soil and conversely, 2 (tomato and cabbage) showed significantly lower CFU/g of-soil in the rhizospbere compared to soil. Both the crop species (in most cases) and the nematode population did not exert significant effect whereas time was the factor to have an obvious effect on the population densities of P. lilacinum. The reduction in CFU/g of soil compared to initial densities ranged from 6.5% in brinjal to 14.'6% in cabbage, 9.8% in brinjal to 21.8% in cabbage, and 17.3% in maize to 32% intomato at 10, 20 and 30 DAI. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2015-01-01T00:00:00Z http://localhost:8080/handle/123456789/4320 EDITING STANDARDIZATION ON TEMPERATURE AND TIME FOR HOT WATER TREATMENT OF SELECTED CROPS AGAINST SEED BORNE DISEASES AKTER, MD. BABUL The experiment was conducted at the Central Laboratory of the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, during July 2014 to December 2015. Effect of hot water treatment at different temperatures with varying durations on important seed-borne pathogens of rice, wheat, country bean, tomato and eggplant seeds were studied. Seeds dipping in hot water at 50 to 60° e for 5, 10 and 15 mins were evaluated for standardization of temperature and time for controlling seed-borne pathogens. Hot water treatment at 53/ 54°C for 15 mins gave the highest seed germination (90%) and completely eradicated seed infection by Bipolaris oryzae and Fusarium spp. of rice seed. Wheat seeds dipped in hot water at 51 / 52°e for 10 min yielded the maximum seed germination (90%) and reduced seed infection of Bipolaris sorokiniana and Fusarium spp. For country bean, 55 156°C for 15 mins was found effective with the highest seed germination (85%) and no seed infection of Aspergilus niger. In case of tomato and eggplant seeds 53 / 54°C for 15 mins gave the highest seed germination (88%, 90%, respectively) and no seed infection (0.0%). Hot water treated seeds sown in the pot soil gave 33.75%,200/0,21.5%,22.5% and 20% higher seed germination, respectively for rice, wheat, country bean, tomato and eggplant in comparison to untreated control. Therefore, hot water treatment of specific temperature may be suggested for controlling important seed-borne pathogens of rice, wheat, country bean, tomato and eggplant seeds for enhancing seed germination and reduction of seed infection. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2015-01-01T00:00:00Z http://localhost:8080/handle/123456789/4319 EVALUATION OF TOMATO GENOTYPES AGAINST TOMATO YELLOW LEAF CURL VIRUS (TYLCV) SYDUJJAMAN, SYED MD. Present studies had been conducted to evaluate different varieties and lines of tomato against Tomato yellow leaf curl virus (TYLCV) during the period from October 2014 to March 2015. Five varieties namely BARl Tomato-J, BARl Tomato-Z, BARI Tomato-9, Loc81jessore-2, Local jessore-J and five lines namely BD-7276, BD-7281, BD-7290, BD-7754, BD-7762 were evaluated against Tomato yellow leaf curl virus (TYLCV). The effect of diseases on yield and yield contributing characters were also observed. The tomato varieties and lines differed significantly among themselves in respect of disease incidence. The highest disease incidence was found in Local Jessore-3 (100%), Bd-7281 (100%), BD-7754 (100010) and BD-7762 (100%). The lowest disease incidence was found in the variety BARI Tomato-9 (00%). Considering the performance of selected tomato varieties and lines, it was observed that line BD-7276 was graded as tolerant against Tomato yellow leaf curl virus (TYLCV). The cultivars BARI Tomato-3, BARI Tomato-7, Local Jessore-2 and line BD-7290 showed moderate resistance, while the Jines BD-7762, BD-7754, BD-7281 and Loc81jessore-3 were highly affected by Tomato Yellow Leaf Curl Virus (TYLCV) among different treatments used in the experiment. In case of number of leaves, branch and flowers per plant, the maximum number of leaves, branch and flowers was recorded in the variety BARI Tomato-9 (72.00, 10.67 and 68.33 respectively). The minimum number of leaves, branch and flowers was obtained in the variety Local Jessore-3 (47.67, 6.00 and 51.67 respectively). On the basis of yield and yield contributing characters, the yield performance also differed significantly. The highest yield per plant was recorded in the variety BARI Tomato-9 (2.70 kg) and the lowest in Local Jessore-3 (1.09 kg). In case of the physiological features, we also founded a significant difference among the different varieties and lines. In case of net chlorophyll content, net assimilation rate, intercellular carbon-di-oxide concentration and respiration rate per plant, in 811cases the highest value was recorded in the variety BARI Tomato-9 (65.17,1.03,37.33 and 1.25 respectively) and the lowest was in Local Jessore3 (40.20, 0.33, 16.67 and 0.33 respectively). A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2015-01-01T00:00:00Z http://localhost:8080/handle/123456789/4318 YIELD LOSS ASSESSMENT OF WHEAT DUE TO LEAF BLIGHT DISEASE CAUSED BY Bipolaris sorokiniana NOWRIN, SANTA ISLAM The experiment was conducted in the farm of Sher-e-Bangla Agricultural University and central laboratory of the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207 during the Rabi season from November 2014 to June 2015. The experiment was laid out in a RCBD (one factor) with three replications. Multiple treatment experiment with 11 treatments, viz. To (control); Tl (1 spray with Tilt 250 EC @ 0.1%); T2 (2 sprays with Tilt 250 EC @ 0.1%); T3 (3 sprays with Tilt 250 EC @ 0.1%); T4 (4 sprays with Tilt 250 EC @ 0.1%); r,(5 sprays with Tilt 250 EC @ 0.1%); T6 (6 sprays with Tilt 250 EC @ 0.1%); T7 (7 sprays with Tilt 250 EC @ 0.1%); Ts (8 sprays with Tilt 250 EC @ 0.1%); T9 (9 sprays with Tilt 250 EC @ 0.1%); TIO (10 sprays with Tilt 250 EC @ 0.1%) were applied in the experiment to make the variation in the disease severity and respective yield of treated plot. Different treatment comprising different number of spraying had remarkable influence the disease severity of leaf blight, yield and yield contributing characters of wheat. The lowest (0.0%) percent disease index (PDI) and the highest yield (5.35t1ha) was recorded in case of treatment Tro where 10 spraying were done with Tilt-250 EC @ 0.1%. The highest PDI and the lowest yield were counted in case of treatment To (control). The disease severity and yield was varied significantly on the basis of number of spraying. Using the varied disease severity (PDI) and the corresponding yield the regression equation for yield loss assessment was constructed as Y = 2.58+ 0.40Xi where 0.4 % loss of yield would be incurred for increasing each percent of disease severity (PDI). A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2015-01-01T00:00:00Z