http://localhost:8080/handle/123456789/4784 2026-04-18T16:16:57Z 2026-04-18T16:16:57Z ISLAM, SYIDUL http://localhost:8080/handle/123456789/4787 2023-02-01T04:28:26Z 2020-01-01T00:00:00Z

COLIFORM AND STAPHYLOCOCCAL MASTITIS IN COWS RISK FACTORS AND TRENDS IN ANTIMICROBIAL RESISTANCE ISLAM, SYIDUL This study was undertaken to know the prevalence of E coli & Staphylococcus aureus causing mastitis. Total Prevalence of mastitis was determined from different farms of Amtali, Barguna. Identification of different bacteria from positive samples were performed by cultural characteristics, and biochemical tests to some extent. The overall prevalence of mastitis was 5%. The prevalence of mastitis in cross breed cow was 6.42% and in local cow 3.75%. Prevalence of mastitis was higher in older animal of 7-8 years old. The prevalence of mastitis in 3-4, 5-6, 7-8 and 9-10 year old cows were 3.08%, 4.29%, 5.88% and 5%, respectively. The higher number of mastitis incidence was 8.75% during 3 rd- 4 th parity than 3.33% during 1 st -2 nd parity and 3.84% when ≥5 parity. Among 15 mastitis infected cattle, the prevalence of mastitis in cow had peri-parturient disease were 86.67% and cows without histories of peri-parturient disease were 13.33%. Among 15 mastitis infected cattle, the prevalence of mastitis were 26.67% at 1st-2 month of lactation 53.33%, at 3 rd -4 th month of lactation and 20% and at 5 th -6 th month of lactation respectively. The prevalence of mastitis in dry and wet season was 33.33% and 66.67% respectively. The occurrence of mastitis was 26.67% in cows in farms with brick-block floor and 20% in cows in farms with soil floor. Only 53.33% cows were affected with mastitis when the floor was wet and soiled. The occurrence of mastitis had relation with the cleanliness of farm. Among 15 mastitis infected cattle, 73.33% infected cow were reared in dirty farm and 26.67% infected cows are reared in clean farm. The prevalence of E. coli and S. aureus in mastitis milk samples were 73.33% and 66.67% % respectively. Nine antibiotics were used to know the susceptibility, intermediate resistance and resistance percentage against the isolated bacteria. Amoxicillin showed 36.36% sensitivity, Ampicillin showed 36.36%, Tetracycline shows 54.54% sensitivity, Streptomycine and Co-trimethaxole/Trimethoprim showed 81.81% sensitivity, Gentamycin, Cefttriaxone shown 90.91% sensitivity, Cefuroxime and Cefixime shown 100% sensitivity against E. coli. Cefuroxime and Cefixime were highly sensitive and Ceftriaxone and Gentamycin shown 9.09% resistance. Amoxicilin showed higly resistance among these 9 antibiotics. Amoxicilin showed 45.45% resistance. Amoxicillin, Ampicillin and Tetracycline showed 18.18%, 27.27% and 9.09% intermediate resistance respectively against E. coli. Against S. aureus; Cotrimethaxole/Trimethoprim showed 60% sensitivity, Tetracycline showed 70% sensitivity, Amoxicillin, Ampicillin, Streptomycine showed 80% sensitivity, Gentamycin, Cefttriaxone shown 90% sensitivity, Cefuroxime and Cefixime showed 100% sensitivity. It was observed that Cefuroxime and Cefixime we r e highly sensitive and ceftriaxone showed 10% resistance Cotrimethaxole showed higly resistance among these 9 antibiotics. Cotrimethaxole/ Trimethoprim showed 30% resistance. Amoxicillin, Tetracycline, Streptomycine and Cotrimethaxole/Trimethoprim showed 10% intermediate resistance against S. aureus. This study showed that antibiotic resistance against E. coli and S. aureus was increased. So, prevention and conrol of the outbreak of this disease is very necessary through good therapeutic management and hygienic care of the farm and dairy cow. A Thesis Submitted to Department of Medicine & Public Health Sher-e-Bangla Agricultural University, Dhaka-1207 In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN MEDICINE

2020-01-01T00:00:00Z KHAN, MD. ROKNUZZAMAN http://localhost:8080/handle/123456789/4786 2023-02-01T04:26:03Z 2020-01-01T00:00:00Z

MICROBIOLOGICAL AND PHYSICOCHEMICAL ANALYSES OF SUPPLIED WATER IN SELECTED DAIRY FARMS OF DHAKA CITY KHAN, MD. ROKNUZZAMAN This study aimed to assess the quality of supplied drinking water for dairy farm in selected farms of Dhaka city by considering the microbiological and physicochemical parameters. The study areas comprised of 5 different locations in Dhaka city namely 60 feet area, Mirpur-1, Mirpur-2, Kalshi and Sher-e-Bangla Agricultural University (SAU) campus area. Samples were collected randomly from each site in four replicates. Microbiological analyses were conducted in laboratory of Medicine and Public Health (MEPH), SAU and physicochemical analyses were conducted in ACI Animal Health Diagnostic Laboratory. Load of coliform bacteria, E. coli, Balantidia and Giardia were taken into account under microbiological parameter. pH level, concentration of TDS, hardness, chlorides and iron were analyzed for physicochemical parameters. The study showed that the prevalence of coliform bacteria and E. coli were 100% and the bacterial load were higher than the maximum safety limit for cattle in all study area. Among those sites, concentration of coliform (2.63×10 8 ) and E. coli (7.05×10 7 ) were highest in the water of Mirpur-1, and lowest in SAU campus area. However, no Giardia and Balantidia were present in these samples of these study area. The physicochemical studies showed that water samples of 60 feet area held the highest concentration of TDS (397.00 ± 6.27) and chloride (0.95 ± 0.06), and lowest pH value (6.13±0.05). Water of all study sites contained higher iron concentration than recommended safety margin where Mirpur-1 contained highest concentration of iron (0.97 ± 0.04) while Mirpur-2 contained lowest (0.78 ± 0.02). The water of Mirpur-2 contained highest value of pH (6.70±0.14) and hardness (165.00 ± 2.45). The water samples of kalshi showed lowest concentration of chlorides (0.52 ± 0.02) and hardness (75.00± 4.08). The water of SAU cams area held lowest level of TDS (307.50 ± 6.46). Concentration of all physicochemical quality factors (pH, TDS, hardness, chlorides) lied within the expected ranges except iron. Compendium of this study disclosed that the supplied water quality of dairy farm in selected area of Dhaka city is good apart from higher bacterial load (Coliform and E. coli) and iron concentration which have inverse impact on health and production of cattle. Consequently, water quality of SAU campus area is comparatively better and Mirpur-1 is comparatively poorer than other study areas. This study recommended that water of Dhaka city should be treated for minimizing the iron concentration and good water management should be exerted to reduce the bacterial contamination for enhancing better health and performance of dairy farm in Dhaka city. A Thesis Submitted to The Department of Medicine and Public Health, Faculty of Animal Science and Veterinary Medicine Sher-e-Bangla Agricultural University, Dhaka In partial fulfillment of the requirements for the degree of MASTERS OF SCIENCE (MS) IN MEDICINE

2020-01-01T00:00:00Z ISLAM, MD. KHAIRUL http://localhost:8080/handle/123456789/4785 2023-02-01T04:23:48Z 2020-01-01T00:00:00Z

ISOLATION, IDENTIFICATION AND ANTIBIOTIC SENSITIVITY PROFILING OF ESCHERICHIA COLI AND SALMONELLA SPP. FROM DEAD BROILER IN LOCAL MARKET OF DHAKA CITY ISLAM, MD. KHAIRUL This study was carried out in Sher-e-Bangla Agricultural University, Dhaka for isolation, identification and antibiotic sensitivity profiling of Escherichia coli and Salmonella spp. from dead broiler in local market of Dhaka city. A total of 150 liver and heart samples were collected from dead broiler. The microorganisms of Escherichia coli and Salmonella spp. were isolated and identified. Samples were collected aseptically and inoculated onto various culture media for isolation of Escherichia coli and Salmonella spp. Identification of bacteria from positive samples was performed by cultural characteristics and biochemical tests. Hundred percent prevalence with microorganism was found from all the samples. The prevalence of E. coli and Salmonella spp. in liver samples was (45.33%) and (40%) and in heart samples it was (44%) and (38.67%) respectively. The overall prevalence of E. coli and Salmonella spp. in broiler was (44.67%) and (39.33%). Antibiotic sensitivity profiling of the isolated E. coli and Salmonella spp. was performed by the disc diffusion method against 10 commonly used antibiotics. The highest rate of sensitivity against E. coli was found with Ceftriaxone (52.24%) followed by Gentamicin (49.25%) and Streptomycin (44.78%). The highest rate of resistance was recorded in Tetracycline (52.24%) followed by Ampicillin (50.75%), Cefuroxime (47.76%), Amoxycillin (46.27%) and Co-Trimoxazole (46.27%). The highest rate of antibiotic sensitivity against Salmonella spp. was found with Ceftriaxone (52.54%) followed by Gentamicin (50.85%) and Streptomycin (47.46%). The highest rate of resistance of Salmonella was recorded in Cefuroxime (54.24%) followed by Ampicillin (52.54%), Cefixime (50.85%), Tetracycline (49.15%), Amoxycillin (47.46%) and Co-Trimoxazole (45.76%). Based on the present study, it may be concluded that Ceftriaxone should be best result against both E. coli and Salmonella spp. followed by Gentamicin and Streptomycin under the present study. A Thesis Submitted to the Department of Medicine & Public Health Sher-e-Bangla Agricultural University, Dhaka In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN MEDICINE

2020-01-01T00:00:00Z