http://localhost:8080/handle/123456789/4300 2026-04-18T16:17:50Z 2026-04-18T16:17:50Z HASAN, MD. JUWEL http://localhost:8080/handle/123456789/4735 2023-01-09T04:29:21Z 2021-12-01T00:00:00Z

MOLECULAR AND HISTOPATHOLOGICAL STUDIES OF WHITE SPOT SYNDROME VIRUS INFECTION IN TIGER SHRIMP (Penaeus monodon) HASAN, MD. JUWEL Since the 1980s, shrimp farming has been rapidly expanded and has become the third largest export earnings source, contributing about 3.57% to national GDP of Bangladesh. Now this sector facing a serious problem with a disease named White Spot Disease (WSD) caused by dreadful White Spot Syndrome Virus (WSSV), which leads to 100% mortality in the shrimp farm within 3-5 days. This study showed PCR detection and prevalence rate of WSSV infection in Penaeus monodon and further confirmed by histopathological study to observe the pathological changes in the tissue. The prevalence of this virus has been studied in six selected farms located in the Bagerhat and Satkhira districts of Bangladesh. Both one-step and two-step PCR were used to detect using two pairs of primers, namely, 146F1/146R1 and 146F2/146R2, amplifying the 1447 bp and 941 bp fragments, respectively. Out of 36 randomly selected shrimp samples, 25 samples were WSSV-positive other 11 samples were negative by one-Step PCR. The negative eleven samples were further subjected to two- step PCR and resulted only 3 samples being negative. The prevalence rates of WSSV infection were 83.33% and 55.56% in Satkhira and Bagerhat district, respectively, confirmed by one-step PCR and the prevalence rate of WSSV infection was 83.33% and 100% in Satkhira and Bagerhat district, respectively, confirmed by two-step PCR. However, the overall prevalence rate increased from one-step to two-step PCR from 69.44% to 91.67%. Between two methods two-step PCR was more efficient than one-step PCR. The shrimp specimens showing clinical signs of WSSV were positive by both one-step and twostep PCR. Some of the apparently healthy samples were also found to be positive for WSSV by two-step PCR. Histopathological studies of hepatopancreas of two-step PCR confirmed WSSV-positive samples resulted in degenerated tubule lumen, degenerated and sloughing hepatopancreas with necrosis and infected muscle tissue resulting in fragmentation and separation of muscle fibers. Furthermore, it is suggested that more molecular studies about WSSV and disease management is required to increase the shrimp production in Bangladesh. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY SEMESTER: JULY-DECEMBER, 2021

2021-12-01T00:00:00Z HOSSAN, MOHAMMAD IQBAL http://localhost:8080/handle/123456789/4731 2023-01-09T04:18:30Z 2021-12-01T00:00:00Z

EFFECTS OF DIETARY SUPPLEMENTATION OF Sargassum vulgare ON GROWTH AND HEMATOLOGICAL PARAMETERS OF ORNAMENTAL FISH KOI CARP (Cyprinus rubrofuscus) HOSSAN, MOHAMMAD IQBAL A research was conducted to assess different formulated diets supplemented with different doses of seaweed (Sargassum vulgare) on ornamental fish Koi carp (Cyprinus rubrofuscus) in the aquaculture laboratory of Department of Aquaculture, Sher-e-Bangla Agricultural University to evaluate the growth and hematological parameters. Total four treatments were applied including Treatment- 1 (Control, 0% seaweed), Treatment-2 (5% seaweed), Treatment-3 (10% seaweed) and Treatment-4 (15% seaweed) each with three replicates. Four iso-proteinase feed was formulated where average percentage of protein, lipid, fiber and carbohydrate in formulated diet were 27.59%, 6.1%, 7.59 and 48.96%. The initial mean weight of fishes was 14.4±2.12, 14.5±0.75, 13.1±2.4 and 12.8±1.43 g in T-1, T2, T-3 and T4 respectively. The experiment was concluded in 100L glass aquarium with mechanized water filter system for each for 8 weeks and weekly sampling were done. Feed was applied twice a day. Temperature, pH, TDS, NH 3, NO 2 - ii and NO 3 - data were collected daily during the experimental period and there was no significant difference in water parameters among treatments. Mean weight (g) of fish, length (cm) and width (cm) data were collected weekly basis. The mean weight gain was found 7.13±1.84, 8.06±1.58, 6.98±1.84 and 4.72±0.52 in T-1, T-2, T-3 and T-4 respectively. The highest weight gain was found in T-2 (5% seaweed) and the percentage of weight gain 55.86% found in the same treatment. There was no mortality found in T-2 and T-4. Survival rate were found 96.6% in Control. The lowest FCR 1.43±0.26 found in T-2 and SGR also found highest in the same treatment. Hematological parameter showed that blood hemoglobin and glucose level was higher in T-2 than others treatments which were 11.6±0.17gm/dl and 6.33±0.26 mmol/l. Serum protein was found 6gm/dl in T-2 and lowest value was found in control. Spleen Somatic Index (SSI) percentage of experimental fishes was also found higher in T-2 and lowest one was recorded in T-1. Refractive index (RI) of blood serum was found better in T-2 fishes compared to other treatments. It can be concluded that feed supplemented with 5% seaweed (T-2) diet showed better growth performance and hematological parameters compared to other treatments. Feed supplemented with brown seaweed Sargassum vulgare as a dietary ingredient can be used for better immunity and growth performance of ornamental fish. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY SEMESTER: JULY-DECEMBER, 2021

2021-12-01T00:00:00Z IRTIZA, MD. HASIN http://localhost:8080/handle/123456789/4727 2023-01-09T04:09:35Z 2021-12-01T00:00:00Z

BIOINFORMATIC ANALYSIS OF MITOCHONDRIAL GENOME OF HUMPED FEATHERBACK CHITAL FISH, Chitala chitala (HAMILTON, 1822) IRTIZA, MD. HASIN The present study was about the bioinformatic analysis of mitochondrial genome of an endangered featherback fish, Chitala chitala. Although Chitala chitala plays significant role in inland fishery, but its’ evolution has received little attention. In the current study, the mitogenome of Chitala chitala was retrieved and investigated. Some of the bioinformatics tools were applied to measure important gene parameters including AT/GC-skewness, codon adaptation index (CAI), the effective number of codons (ENc) and GC percentages of each protein coding gene. The majority of AT-skew and GC-skew values of all protein coding genes of the species were negative, and the amplitude of the GC-skew was larger than the AT-skew. On the basis of comparative selective strength analysis with the PCGs of two related mitogenomes, I discovered that most of the PCGs retained the Ka/Ks ratios less than one implying that they evolved under strong negative or purifying selection. Moreover, I analyzed the codon frequency and relative synonymous codon usage (RSCU). Then, I identified a total of 22 RSCU values (>1) and revealed 6 codons as “over-presented” that implied for codon usage bias to engage in highly expressed genes for efficient protein synthesis via translational selection. The phylogenetic tree and time tree were constructed by Maximum Likelihood (ML) method, providing further supplement to the evolution of the fish. Regarding on the facts of the presence of codon usage biasness rolling in translational selection and the signs of purifying selection identified in PCGs indicate obvious conservation of this endangered fish species. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY SEMESTER: JULY-DECEMBER, 2021

2021-12-01T00:00:00Z NAYEEM, DIDER HAIDER http://localhost:8080/handle/123456789/4726 2023-01-09T04:00:47Z 2021-12-01T00:00:00Z

ASSESSMENT OF FECUNDITY, HATCHING & SURVIVAL RATE OF NILE TILAPIA (Oreochromis niloticus) APPLYING FEED WITH GARLIC AND GINGER EXTRACT NAYEEM, DIDER HAIDER The study was conducted at commercial hatchery at BRAC Tilapia Hatchery, Magura, Bangladesh and the analytical studies were conducted at Aquaculture Laboratory, Sher-e-Bangla Agricultural University to evaluate the effect of Garlic and Ginger extract in Nile tilapia diets on their reproductive performance (fecundity and hatching), survival rate on produced fry and hematological parameters of fry. A treatment was used as control without any extract. Nile tilapia (O. niloticus) with average weight of 267 g ± 3.16 were stocked for spawning into nine hapas at a density of 13 fish (10 females and 3 males) per 10 sq meter hapa, Three hapas with same diameters were used as replicates for each treatment. Hapas were fixed in an earthen pond (5 decimals). Feeding was done at a rate of 0.5% from their total biomass. Water quality parameters (temperature, pH, dissolved oxygen, ammonia) were measured weekly. Production of seed (eggs and fry) was compared with the tested diets. The highest mean of egg number (132.05 pcs) & mean spawn number (98.29 ± 1.11 pcs) per gram were obtained with the diets contained 0.02% Garlic extract (Treatment 2). Survival rate (84.24±0.05%) of fry was also found best with Treatment 2. Tilapia diets supplemented with Garlic extract had best responded on their reproductive performance (Hatching 80.63% ±1.05) and highest value FCR (1.43±0.19) & SGR (1.50 ± .19). The garlic extract treatment showed excellent performance for all the parameter under studied. Hence, the feeding with (0.02%) garlic extract can be used for large scale in tilapia breeding. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY SEMESTER: JULY-DECEMBER, 2021

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