http://localhost:8080/handle/123456789/3224 2026-04-18T16:17:02Z 2026-04-18T16:17:02Z ALAM, MD. KHAIRUL http://localhost:8080/handle/123456789/3590 2021-12-22T08:06:53Z 2020-01-01T00:00:00Z

BIOFLOC CULTURE METHOD FOR GROWTH PERFORMANCE, SURVIVAL RATE AND NUTRITIONAL COMPOSITION OF SNAKEHEAD FISH (Channa striata) ON NATURAL EXTRACT SUPPLEMENT ALAM, MD. KHAIRUL Snakehead fish (Channa striata) is a well known and important freshwater fish in Southeast Asian countries. The experiment was conducted at Sher-e-Bangla Agricultural University Biofloc Culture Lab to evaluate the growth performances, survival rate and nutritional composition of snakehead fish (C. striata). There has four treatments with three replications. Water quality parameters were monitored through temperature, dissolved oxygen, pH, ammonia, total dissolved solids, alkalinity and hardness. Maximum length increase (14.15±0.2) cm of C. striata was found in treatment-4. Highest weight gain also noticed in the same treatment which was (105.67±6.65)g. Control treatment showed lowest performance among the parameter studied. Maximum specific growth rate was 2.70% in treatment-4 whereas 2.50% was minimum in treatment-1. In treatment-3 survival rate was highest, which was 75.76% and in control treatment, the lowest survival rate was recorded that was 47.88%. Feed conversion ratio was better (1.08) in tretment-4. Daily mean weight gain was noteworthy in treatment-4 which was 1.50g and lowest daily mean weight gain was recorded 1.1g in control treatment. From the analysis of proximate composition of C. striata 69.67% protein content was available in treatment-4 which was maximum and the lowest protein content was found 61.84% in treatment-2. Considering all the results, the optimum growth performance and nutritional composition was better in treatment-4 (Extract of Garlic and Amla) compared to other treatments. In the era of aquaculture and microbial biotechnology there has a huge potentiality to fulfill the requirement of fish protein by the culture of C. striata in biofloc technology. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY

2020-01-01T00:00:00Z ISLAM, MD. SYFUL http://localhost:8080/handle/123456789/3589 2021-12-22T08:03:12Z 2020-01-01T00:00:00Z

ASSESSMENT OF HERBAL EXTRACT FOR GROWTH AND NUTRITIONAL QUALITY OF JUVENILE FRESHWATER PRAWN (Macrobrachium rosenbergii) UNDER BIOFLOC SYSTEM ISLAM, MD. SYFUL The study was conducted in the Biofloc Lab, Department of Aquaculture, Sher-eBangla Agricultural University to assess the potentiality of Macrobrachium rosenbergii, growth performance and its nutritional quality in Biofloc Technology (BFT) from July to November, 2020. Total five treatments were applied including T1 (Control), T-2 (Extract of Ginger), T-3 (Extract of Amla), T-4 (Extract of Garlic) and T-5 (Extract of Garlic and higher stocking) with three replications. The initial mean stock weight of prawn were 3.14±0.19, 3.04±0.27, 3.26±0.16, 3.36±0.06 and 3.02±0.36g in T-1, T-2, T-3, T-4 and T-5 respectively. After completing the research duration, the highest mean weight gain and percentage weight gain were 10.9±0.3g and 297.8±16.2% noticed in the T-3. The survival rate was maximum (99.5±0.8%) in the control treatment. The best FCR and SGR were 1.2±0.1 and 2.1% also noticed in the T-3. During the research 35% protein containing commercial feed was given and floc was produced at balanced level which was found 21.7% protein. From the proximate composition analysis, the highest protein percentage of prawn 74.13±.68% was found in the T-3. The best growth performance and nutritional value was observed in T-3 (Extract of Amla) compared to other treatments and also tells the immense possibility of M. rosenbergii culture in biofloc in the era of aquaculture and microbial biotechnology for mitigating the demand of animal protein. A Thesis Submitted to The Department of Biotechnology, Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka-1207 In partial fulfilment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY

2020-01-01T00:00:00Z CHAKRABORTY, NARAYAN http://localhost:8080/handle/123456789/3588 2021-12-22T08:00:50Z 2020-01-01T00:00:00Z

IN VITRO REGENERATION OF DRAGON FRUIT (Hylocereus undatus) CHAKRABORTY, NARAYAN The present experiment was carried out in the Laboratory of Biotechnology, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka1207 during the period of February, 2020 to December, 2020 to study the effect of BA (1.0, 1.5, 2.0, 2.5 and 3.0 mg/l), NAA (0.25, 0.50, 0.75 and 1.00 mg/l) individually or in combination of both for in vitro regeneration of Dragon fruit. The shoot tips of Dragon plants were used as explants which were sterilized with 70% ethanol and 0.1% HgCl 2 . The highest percentage of shoot induction (76.00%) was observed in MS medium with BA 2.0 mg/l in minimum 12.2 days. The maximum number of shoot (3.20) and the highest shoot length (4.02 cm) were obtained at 42 days with the treatment BA 2.0 mg/l and BA 2.50 mg/l respectively. The treatment NAA 0.50 mg/l produced the highest percentage of root (68.00%) and minimum 12.40 days required to produce root with the treatment NAA 0.25 mg/l. The maximum number of root (5.40) and the highest length of root (5.38 cm) at 42 days were recorded with the treatment NAA 0.50 mg/l and NAA 0.25 mg/l respectively where the control treatment gave the lowest result. In combined effect, BA 2.0 mg/l+ NAA 0.50 mg/l showed the highest percentage of shoot (86.67%) and root (93.33%) in 11.67 days and 12.67 days respectively. The maximum number of shoot (3.67) and the highest shoot length (4.36 cm) were obtained at 42 days in BA 2.0 mg/L+ NAA 0.50 mg/l. The maximum number of root (5.67) and the highest length of root (6.27 cm) at 42 days were recorded with BA 2.0 mg/l+ NAA 0.50 mg/l where the treatment BA 1.0 mg/l + NAA 0.25 mg/l showed the lowest result. Finally, the survival rate of in vitro regenerated plantlets was recorded 84% in natural condition. Overall, feasible protocol has been developed for in vitro rapid regeneration of Dragon fruit having potential application in large scale multiplication for commercial purpose. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY

2020-01-01T00:00:00Z AFROZ, TANIA http://localhost:8080/handle/123456789/3587 2021-12-21T06:56:50Z 2020-01-01T00:00:00Z

MICROPROPAGATION OF BLACK PEPPER (Piper nigrum L.) AFROZ, TANIA The present study was undertaken in the Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e- Bangla Nagar, Dhaka-1207, during the period from January to September, 2020 to evaluate the effect of Benzyl adenine (BA) Indole-3-acetic acid (IBA) on in vitro regeneration of Black pepper. The nodal segments of Black pepper were used as explants which were inoculated in MS media supplemented with different conc. (0.5, 1.0, 1.5 and 2.0 mg/L) of BA and IBA (0.5, 1.0, 1.5 and 2.0 mg/L) either alone or in combination of BA (1.0, 1.5, 2.0 and 2.5 mg/L) and IBA (1.0, 1.5, 2.0 and 2.5 mg/L). The experiments were arranged in Completely Randomized Design (CRD) with five replications. The maximum shoot induction was obtained with 2.0 mg/L BA.The highest number of leaves were recorded in the treatment 2.0 mg/L BA at 8 WAI. The maximum number of root was regenerated in the treatment 2.0 mg/L IBA. The combination of both the hormone 2.0 mg/L BA + 2.0 mg/L IBA showed best result for shoot induction, shoot length and also for total number of leaf. Maximum percentage of root induction and root length also found in the same treatment. Regenerated plantlets survive 46.67% in shade house and 57.14% in open atmosphere. Therefore, an efficient protocol has been developed for in vitro regeneration of Black pepper which can be used for large scale production of Black pepper. A Thesis Submitted to the Dept. of Biotechnology Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY

2020-01-01T00:00:00Z