January, 2016 (Vol. 1, No. 1)

Isolation, Characterization and Antibiogram Study of Pasteurella multocida Isolated from Ducks of Kishoreganj District, Bangladesh

2022-12-12T10:51:19Z

Isolation, Characterization and Antibiogram Study of Pasteurella multocida Isolated from Ducks of Kishoreganj District, Bangladesh Kamruzzaman, M; Islam, M; Hossain, MM; Hassan, MK; Kabir, MHB; Sabrin, MS; Khan, MSR A study was carried out to isolate and characterize Past eurel l a mul t oci da isolates from ducks with emphasis on antibiotic sensitivity and resistant pattern. A total of 100 fecal samples were aseptically collected from sick ducks during suspected duck cholera outbreak in Kishoreganj district, Bangladesh during the period from January to December, 2009. The processed samples were cultured on blood agar media for isolation of bacteria. Pure cultures were obtained after subsequent cultures. P. mul t oci da isolates were identified according to their morphology, staining, motility, cultural and biochemical properties. The isolated bacteria were then subjected to antibiotic sensitivity test. The prevalence of isolated P. multocida from ducks was 34%. From cultural studies it was found that P. multocida showed small, glistering, mucoid and dew drop like colony on blood agar plate. They did not grow on MacConkey's agar and found to be non-haemolytic on blood agar plates. The isolates were found Gram negative with coccobacillary appearance, bipolar organisms and non-motile. All the isolates were found positive to catalase, oxidase, indole and nitrate reduction tests and negative to methyl red, Voges-Proskaur, citrate utilization and H S production tests. The isolates fermented dextrose, sucrose and manitol without production of gas but did not ferment lactose and maltose. Isolated P. multocida isolates were sensitive to ci pr of l oxaci n ( 95%) and azithromycin (80%), intermediate sensitive to gentamycin (85%), tetracycline (75%), amoxicillin (75%) and erythromycin (70%), but resistant to penicillin G (100%). 2

Use of black cumin in layer diet as cholesterol lowering agents in egg yolk

2018-11-29T10:42:59Z

Use of black cumin in layer diet as cholesterol lowering agents in egg yolk Hossain, MM; Asaduzzaman, M..; Asad, L; Akter, M; Rahman, ANMI This study was conducted to investigate the effects of different levels of black cumin seeds (Nigella sativa L.) on egg production and cholesterol concentration in egg yolk of laying hens. A total of 60 commercial layer strain day old layer chicks were collected and divided into three groups treated with 1.0%, 1.5% and 2.0% black cumin inclusion. The concentrations of total lipids, total cholesterol, phospholipids and triacylglycerols in serum and egg yolk were measured. Feeding of the diets with 1%, 1.5% and 2% black cumin seeds during the laying period found egg yolk cholesterol by 11.12, 9.88 and 9.83 mg/g respectively. The results found that feed efficiency ratio, egg production, body weight, feed intake and egg weight were nonsignificant between the treatments. However, egg yolk cholesterol concentration was found that 1.5% and 2.0% black cumin in diet were reduced cholesterol concentration insignificance (P<0.05). So, dried black cumin supplementation in diets had no any adverse effect on egg production and egg weight. Furthermore, egg yolk cholesterol concentrations were decreased. Hence, it is concluded that black cumin (Nigella sativa L.) seeds and/or the active principle are of interest as potential egg-yolk cholesterol-lowering agents.

Effect of Different Dietary Levels of Energy on the Growth Performance and Meat Yield of Indigenous Chicken Reared in Confinement under the Rural Condition of Bangladesh

2018-11-29T10:39:20Z

Effect of Different Dietary Levels of Energy on the Growth Performance and Meat Yield of Indigenous Chicken Reared in Confinement under the Rural Condition of Bangladesh Miah, MY; Chowdhury, SD; Bhuiyan, AKFH An experiment was carried out to investigate the effects of different dietary levels of energy on growth performance and meat yields of indigenous (desi) chickens up to the target weight of 950 g in rural condition. One hundred indigenous unsexed chicks aged 4 weeks were considered for the feeding trial until the body weight reached at 950g. Chicks were divided into two dietary treatments having five replications (10 chicks per replication) and reared on littered floor in an open sided house by providing 0.092 m² floor space per bird. Two iso-nitrogenous diets (23% CP) differing in energy contents were formulated to constitute dietary treatments. Diets for comparison were: moderate energy density (MED): ME 2800 kcal/kg and high energy density (HED): ME 3000 kcal/kg. The contents of CP, ME, Calcium (Ca) and total Phosphorous in MED diet were fitted with the requirements of Bureau of Indian Standard (BIS 1992). Body weight, feed intake, FCR and survivability had no significant effect between the two dietary groups during a rearing period of 3-14 weeks. Similarly breast, drumstick, thigh, wing, neck, liver and head weight had no significant effect between the two dietary groups. Profit per bird increased slightly with increasing level of the dietary energy densities. It was concluded that a nutrient density of 3000 ME kcal/kg and 23% CP would be enough to optimize growth rate and FCR of indigenous (desi) chickens in rural condition.

Effect of Vegetable Tanning By-product on the Growth Performance of Caged Nile Tilapia (Oreochromis niloticus) as a Fishmeal Replacement

2018-11-29T10:33:03Z

Effect of Vegetable Tanning By-product on the Growth Performance of Caged Nile Tilapia (Oreochromis niloticus) as a Fishmeal Replacement Newaz, AW; Alam, MJ; Hossain, MM The effect of vegetable tanning by-product (VTBP) on the growth performance of Nile tilapia (Oreochromis niloticus, Linnaeus) under cage culture system was evaluated. The chemical composition of vegetable tanning by-product (VTBP) and Fishmeal (FM) were analyzed. The dry matter (DM), crude protein (CP), ether extracts (EE) and ash content of VTBP were found 90.63, 77.02, 2.83 and 7.19%, respectively. The dry matter content of Fishmeal was recorded 87.3%. Ether extract content of Fishmeal (4.3%) was found slightly higher than the VTBP. The ash content of fishmeal was found 29.5%. But protein content of the Fishmeal (51.9%) was found much lower than the VTBP. Amino acid profile of both VTBP and Fishmeal were analyzed where the former showed higher amounts of some essential amino acids. The presence of aflatoxin in VTBP was found to be negative. The average temperature of pond water recorded during experimental period was 28.37, 27.69 and 28.64°C for treatment 1, 2 and 3 respectively. Dissolve oxygen level was similar to all the treatments and ranged from 6.1 to 6.7 ppm. The pH value was found between the ranges of 7.3 to 7.6. However, there was no significant difference among the survival rate, temperature, dissolved oxygen and pH. The fishes under various feeding treatments showed some difference in growth performance. The initial live weights of fishes were average 3 gm fish -1 . The fish productions in cage were 8.8 kg 10.2 kg 12.9 kg in treatment 1, 2 and 3 respectively. The production showed that treatment 3 significantly different from other treatments. Treatment 3 (VTBP) showed the highest production (12.9 kg/cage) and Treatment 1 obtained the lowest production (8.8 kg cage -1 ). Mean weight gain and daily weight gain of fishes also showed similar results where the highest value for Mean weight (177.6 ± 6.65 g) gain and Daily weight gain (2.9 ± 0.11 g) were recorded in treatment 3. The survival rate was more or less similar to all the treatments which were around 97%.